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Glucose oxidase electrode, preparation method and application thereof

A glucose oxidase and electrode technology, applied in the direction of material analysis, measuring devices, instruments, etc. by electromagnetic means, to achieve the effects of simple and fast preparation process, increased enzyme loading, and large specific surface area

Inactive Publication Date: 2015-06-24
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, these biosensors need to be further improved in terms of enzyme loading, sensitivity, detection range, response time, and anti-interference on the conductive polymer per unit volume of the electrode. For this reason, it is necessary to further improve the specific surface area and Enhanced ion diffusion and direct electron transfer performance

Method used

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  • Glucose oxidase electrode, preparation method and application thereof
  • Glucose oxidase electrode, preparation method and application thereof
  • Glucose oxidase electrode, preparation method and application thereof

Examples

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preparation example Construction

[0021] According to the preparation method of the glucose oxidase electrode of the present invention, the template-free electrochemical method comprises: at a current density of 0.01-1 mA cm -2 , the energization time is 20 minutes to 2 hours, and the temperature of the electrolyte is 20-40°C, the conductive electrode substrate is used as the working electrode, the platinum sheet is used as the counter electrode, and the saturated calomel electrode is used as the reference electrode. The electrolytic solution containing the conductive polymer monomer and the dopant is electrolyzed; preferably, the current density is 0.01-0.5mA·cm -2 , the power-on time is 30 minutes-1 hour, and the temperature of the electrolyte is 25-35°C.

[0022] According to the preparation method of the glucose oxidase electrode of the present invention, the preparation method of the electrolytic solution containing the conductive polymer monomer and the dopant is not particularly limited, and can be prep...

Embodiment 1

[0032] This example is used to illustrate the glucose oxidase electrode of the present invention and its preparation method.

[0033] 1) Preparation of aniline monomer electrolyte: to 20mL with a concentration of 1mol L -1 HClO 4 Add aniline monomer (Sinopharm Group Chemical Reagent Co., Ltd., analytically pure) to the aqueous solution to obtain aniline monomer concentration of 0.1mol L -1 aniline monomer electrolyte.

[0034] 2) Preparation of conductive polymer nanoarray electrodes: at 25°C, a polished platinum sheet (Pt) was used as the working electrode, a Pt sheet (15×20mm, 0.2mm) was used as the counter electrode, and a saturated calomel electrode was used as the The reference electrode uses the above-mentioned aniline monomer electrolyte as the electrolyte, adopts the method of constant current, and the current density is 0.01mA·cm -2 , energized for 0.5 hours, a green polyaniline nanowire array was deposited on the surface of the working electrode (see figure 1 SEM...

Embodiment 2

[0039] This example is used to illustrate the glucose oxidase electrode of the present invention and its preparation method.

[0040] 1) Preparation of aniline monomer electrolyte: 0.5mol L to 20mL -1 HClO 4 Add aniline monomer (Sinopharm Group Chemical Reagent Co., Ltd., analytically pure) to the aqueous solution to obtain aniline monomer concentration of 0.5mol L -1 aniline monomer electrolyte.

[0041] 2) Preparation of conductive polymer nanoarray electrodes: at 30°C, a polished platinum sheet (Pt) was used as the working electrode, a Pt sheet (15×20mm, 0.2mm) was used as the counter electrode, and a saturated calomel electrode was used as the The reference electrode uses the above-mentioned aniline monomer electrolyte as the electrolyte, adopts the method of constant current, and the current density is 0.1mA cm -2 , energized for 40 minutes, and deposited a layer of green polyaniline nanowire array on the surface of the working electrode. The diameter of the polyanili...

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Abstract

The invention discloses a glucose oxidase electrode, a preparation method and an application of the glucose oxidase electrode. The glucose oxidase electrode comprises a conductive electrode base material and a conducting polymer nano array layer which is loaded on the conductive electrode base material, wherein the conducting polymer nano array layer is loaded with glucose oxidases. The glucose oxidase electrode has the advantages that due to the fact that the specific surface area of the glucose oxidase electrode is large, capacity of the glucose oxidases on the conducting polymer per electrode unit volume is greatly improved; the glucose oxidase electrode is provided with a highly ordered nano-structure, a transmission path of ions in a solution can be reduced, internal resistance of the electrode is reduced, diffusion and transmission of the ions in an electrode material are facilitated, so that sensitivity, anti-interference performance and response time of a sensor are greatly improved; the low-cost conducting polymer is used as the electrode material, the whole manufacturing process only comprises two simple electrochemical reaction steps, the manufacturing process is simple and fast, and the cost is low.

Description

technical field [0001] The invention relates to a glucose oxidase electrode and its preparation method and application, in particular, to a kind of electrode with large enzyme loading capacity, high sensitivity, wide detection range, short response time and anti-interference performance on the conductive polymer per unit volume of the electrode. A glucose oxidase electrode with a highly ordered conductive polymer nanostructure, a simple process and low cost, and a preparation method and application thereof are disclosed. Background technique [0002] The rise and development of the field of nanomaterials in recent years has brought new opportunities for the advancement of electrochemical biosensors. Due to the unique physical and chemical properties exhibited by materials reaching the nanoscale, nanomaterials have been more and more widely used in the research and development of new high-performance, fast-response biosensors. [0003] Numerous nanomaterials, such as carbon ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 夏霖全保刚魏志祥
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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