Strain for maleic acid whole-cell catalysis synthesis of L-aspartic acid and method

Abstract

The invention discloses a strain for maleic acid whole-cell catalysis synthesis of L-aspartic acid and a method. The strain is named as Escherichia coli delta fumABC delta gltA delta argG / pMA, the preservation number of the strain is CCTCC NO:M 2019171, and the method comprises the construction processes that knocking out fumarase encoding gene, a citrate synthase encoding gene and an argininosuccinate synthase encoding gene in an original strain, meanwhile, aspartase encoding gene mutation N217K-T233R-V367G and maleic cis-trans isomerase encoding gene mutation G8A-G179A are cloned to expression plasmids to form recombinant plasmids, the recombinant plasmids are converted into the strain with knockout genes, and the target strain is obtained. According to the method for genetically engineered bacterium whole-cell catalysis synthesis of L-aspartic acid, the path for catalysis synthesis of L-aspartic acid by adopting maleic acid as raw materials is achieved, the production cost is lowered, efficient catalysis is achieved, and economy is achieved.

Description

technical field

[0001] The invention belongs to the technical field of genetic engineering and fermentation engineering, and specifically relates to a bacterial strain and a method for catalytically synthesizing L-aspartic acid by utilizing maleic acid whole cells. Background technique

[0002] L-Aspartic acid is widely used in medicine, food and chemical industry. In medicine, it is the main component of amino acid preparations; in chemical industry, it can be used as a raw material for the manufacture of synthetic resins, and is widely used in the synthesis of polyaspartic acid, an environmentally friendly material; especially in the food industry, L-aspartic acid is a It is a good nutritional supplement and is also the main raw material for the production of sugar substitute aspartame. Has a good market prospect.

[0003] At present, L-aspartic acid mainly uses fumaric acid as raw material, and is synthesized by biological enzymatic method. There are relatively few stud...

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