Method for preparing low molecular peptide through proteolysis of pumpkin seeds and dregs

A technology of pumpkin seeds and small molecular peptides, which is applied in the direction of fermentation, etc., to achieve the effects of increased edibility, low price, and good hydrolysis time

Inactive Publication Date: 2012-09-05
徐金军 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pumpkin seed meal contains about 50% protein as determined by our company. It has been reported in the literature that it contains a large amount of active substances, but it has a special flavor. It is generally sold as a feed additive at a low price.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0007] Crush the defatted pumpkin seed meal, pass through a 100-mesh sieve, accurately weigh the pumpkin seed meal powder, add an appropriate amount of water to prepare a pumpkin seed meal protein solution with a certain mass concentration, weigh the total weight for the first time, and pretreat at 100°C for 10 minutes. Add an appropriate amount of water to adjust the total weight to maintain the mass concentration of the pumpkin seed meal protein liquid, adjust the temperature to the reaction temperature and stir for 2 minutes, adjust the pH to the reaction pH, add a certain proportion of protease for constant temperature enzymolysis, and continue to stir, while adding dropwise 0.1mol / L NaOH solution is used to keep the pH of the reaction system constant, and the reaction deviation is generally controlled at ±0.11. After the predetermined reaction time is reached, the pumpkin seed meal protein hydrolyzate is heated to 100°C and kept for 10 minutes to lose the enzyme activity.

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PUM

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Abstract

The invention discloses a process method for preparing small molecular peptide through enzymolysis of double enzymes which are simultaneously added by taking defatted pumpkin seeds and dregs as raw materials. With degree of hydrolysis as an inspection index, and double enzymes, namely papain and AS1.398 protease are taken as hydrolytic enzymes, best hydrolysis process parameters are as follows: the mass ratio of the double enzymes is 1:1, the additive amount of the enzymes is 19%, the mass concentration of a substrate is 35mg / mL, the temperature is 50 DEG C, the pH is 7.0, and the hydrolysis time is 5h.

Description

Technical field: [0001] The invention relates to a technology for preparing small peptides by double-enzyme hydrolyzing plant protein, and its action substrate is defatted pumpkin seed dregs. Background technique: [0002] my country is rich in pumpkin seed resources, with an annual output of about 460,000 tons. Generally, our country is used to eating pumpkin seeds directly or exporting them to other countries as raw materials, and the market lacks corresponding high-end products. At present, the development and utilization of pumpkin seeds are mainly used to process them into crispy baked foods, and there are also reports of processing them into beverages and making raw milk using pumpkin seeds. Few people develop and utilize pumpkin seed meal after oil extraction. Pumpkin seed meal contains about 50% protein as determined by our company. It has been reported in the literature that it contains a large amount of active substances, but it has a special flavor. It is generall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 徐金军刘政聂简琪
Owner 徐金军
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