Method for preparing low molecular peptide through proteolysis of pumpkin seeds and dregs

Abstract

The invention discloses a process method for preparing small molecular peptide through enzymolysis of double enzymes which are simultaneously added by taking defatted pumpkin seeds and dregs as raw materials. With degree of hydrolysis as an inspection index, and double enzymes, namely papain and AS1.398 protease are taken as hydrolytic enzymes, best hydrolysis process parameters are as follows: the mass ratio of the double enzymes is 1:1, the additive amount of the enzymes is 19%, the mass concentration of a substrate is 35mg / mL, the temperature is 50 DEG C, the pH is 7.0, and the hydrolysis time is 5h.

Description

Technical field: [0001] The invention relates to a technology for preparing small peptides by double-enzyme hydrolyzing plant protein, and its action substrate is defatted pumpkin seed dregs. Background technique: [0002] my country is rich in pumpkin seed resources, with an annual output of about 460,000 tons. Generally, our country is used to eating pumpkin seeds directly or exporting them to other countries as raw materials, and the market lacks corresponding high-end products. At present, the development and utilization of pumpkin seeds are mainly used to process them into crispy baked foods, and there are also reports of processing them into beverages and making raw milk using pumpkin seeds. Few people develop and utilize pumpkin seed meal after oil extraction. Pumpkin seed meal contains about 50% protein as determined by our company. It has been reported in the literature that it contains a large amount of active substances, but it has a special flavor. It is generall...

AI Technical Summary

Problems solved by technology

Pumpkin seed meal contains about 50% protein as determined by our company. It has been reported in the literature that it contains a large amount of active substances, but it has a special flavor. It is generally sold as a feed additive at a low price.