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Urine detection kit for diagnosing urothelium carcinoma

A detection kit and a technique for urothelial carcinoma are applied in the field of urine detection kits for diagnosing urothelial carcinoma, which can solve the problems of low sensitivity of urine exfoliation cytology, and achieve the effects of high sensitivity and overcoming low sensitivity.

Inactive Publication Date: 2019-07-16
LIAONING NORMAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

[0007] The invention uses Neu5Gc as a marker for the urine detection kit for diagnosing urothelial carcinoma, wherein the capture reagent is an anti-Neu5Gc primary antibody, and the detection reagent is an anti-Neu5Gc secondary antibody. It overcomes the problem of low sensitivity of urine exfoliation cytology, and can be used for early screening of urothelial carcinoma

Method used

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  • Urine detection kit for diagnosing urothelium carcinoma
  • Urine detection kit for diagnosing urothelium carcinoma

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Experimental program
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Embodiment Construction

[0009] The urine detection kit for diagnosing urothelial carcinoma of the present invention includes a capture reagent, a detection reagent, a coating buffer, a washing solution, a color reagent and a termination reagent, and is characterized in that the capture reagent is anti-Neu5Gc- Anti-, the detection reagent is anti-Neu5Gc secondary antibody.

[0010] The anti-Neu5Gc primary antibody is the antibody IgY obtained by immunizing chicken with Neu5Gc, and the anti-Neu5Gc secondary antibody is the antibody IgG obtained by using rabbit anti-chicken.

[0011] experiment:

[0012] (1) Collection of urine samples:

[0013] A 300 mL urine sample was taken in the morning.

[0014] (2) Urinary protein enrichment method:

[0015] Urinary total protein enrichment by filter pump.

[0016] (3) Detection

[0017] ① Coating: Dilute the capture reagent with coating buffer, 100 μL / well, overnight at 4 °C, discard the solution in the well the next day, and wash twice with washing solutio...

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Abstract

The invention discloses a urine detection kit for diagnosing urothelium carcinoma. The urine detection kit comprises capture reagent, detection reagent, coating buffer solution, scrubbing solution, color-producing reagent and termination reagent. The capture reagent is anti-Neu5Gc primary antibody, and the detection agent is anti-Neu5Gc second antibody. The kit disclosed by the invention is high in sensitivity and has the advantage of the urine exfoliocytology, the problem that the urine exfoliocytology is low in sensitivity is overcome, and the kit can be used for the early screening of the urothelium carcinoma.

Description

technical field [0001] The invention belongs to the field of early cancer diagnosis, in particular to a highly sensitive urine detection kit for diagnosing urothelial carcinoma. Background technique [0002] Cancer is one of the common diseases that seriously endanger people's health. Patients in the early stages of cancer hardly have any obvious symptoms. When patients have symptoms and go to the hospital for treatment, the cancer has often developed to the middle or late stage, resulting in the death of many cancer patients due to missed opportunities for treatment. [0003] Urothelial cancer includes renal pelvis cancer, bladder cancer, ureter cancer, etc. Currently, the most commonly used clinical examination methods are urine exfoliation cytology and cystoscopy. Urine exfoliation cytological examination is carried out with a kit, which is convenient to operate and less painful for the patient. The disadvantage is that the sensitivity is low and is greatly affected by s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/6854G01N33/57407
Inventor 李庆伟逄越滕洪明
Owner LIAONING NORMAL UNIVERSITY
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