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Acer fabri cluster bud inducing and rooting technology

A technology of bud induction and technology, which is applied in the field of bud induction and rooting of red-winged maple, can solve the problems of mass production, unstable technology, and variation of cluster buds, and achieve the effect of retaining excellent traits

Inactive Publication Date: 2019-07-23
福建省龙岩市林业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a technique for induction and rooting of red-winged Acer cluster buds with stable and reliable technology, high production efficiency and mass production, so as to solve the problem of variation and technical instability in the cluster buds induced by tissue culture of Red-winged Acer in the prior art And the problem of not being able to mass produce

Method used

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Examples

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Effect test

Embodiment 1

[0011] The induction and rooting technology of red-winged maple cluster buds of the present invention comprises three stages of explant induction, cluster bud proliferation cultivation and rooting cultivation, and the specific operation steps are as follows:

[0012] (1) Explant induction: take the young shoots of the year and cut off the leaves, rinse them in running water for 1-2 hours, and then soak them in washing powder solution; Disinfect with 75% alcohol for 60 seconds, then sterilize with 0.1% mercuric chloride for 10 minutes, and finally wash with sterile water for 4 times; cut 1 cm budded stem section and inoculate it in induction medium MS+0.5mg / L 6BA +0.3mg / L NAA, placed in the culture room for 30 days to induce culture of axillary buds;

[0013] (2) Proliferation culture of clustered buds: Select sterile axillary buds that have been induced to germinate and inoculate them in the proliferation medium 3 / 4MS+0.4mg / L 6BA+0.2mg / L NAA on a clean bench, and place them in...

Embodiment 2

[0017] The induction and rooting technology of red-winged maple cluster buds of the present invention comprises three stages of explant induction, cluster bud proliferation cultivation and rooting cultivation, and the specific operation steps are as follows:

[0018] (1) Explant induction: take the young shoots of the year and cut off the leaves, rinse them in running water for 1-2 hours, and then soak them in washing powder solution; Disinfect with 75% alcohol for 50 seconds, then sterilize with 0.1% mercuric chloride for 10 minutes, and finally wash with sterile water for 3 times; cut 1 cm of budded stem and inoculate it in induction medium MS+0.5mg / L 6BA +0.3mg / L NAA, placed in the culture room for 25 days to induce culture of axillary buds;

[0019] (2) Proliferation culture of clustered buds: Select sterile axillary buds that have been induced to germinate and inoculate them in the proliferation medium 3 / 4MS+0.4mg / L 6BA+0.2mg / L NAA on a clean bench, and place them in the ...

Embodiment 3

[0023] The induction and rooting technology of red-winged maple cluster buds of the present invention comprises three stages of explant induction, cluster bud proliferation cultivation and rooting cultivation, and the specific operation steps are as follows:

[0024] (1) Explant induction: take the young shoots of the year and cut off the leaves, rinse them in running water for 1-2 hours, and then soak them in washing powder solution; Disinfect with 75% alcohol for 65 seconds, then sterilize with 0.1% mercuric chloride for 15 minutes, and finally wash with sterile water for 4 times; cut 1 cm section of budded stem and inoculate it in induction medium MS+0.5mg / L 6BA +0.3mg / L NAA, placed in the culture room for 35 days to induce culture of axillary buds;

[0025] (2) Proliferation culture of clustered buds: Select sterile axillary buds that have been induced to germinate and inoculate them in the proliferation medium 3 / 4MS+0.4mg / L 6BA+0.2mg / L NAA on a clean bench, and place them...

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Abstract

The invention discloses an Acer fabri cluster bud inducing and rooting technology. The Acer fabri cluster bud inducing and rooting technology includes: taking a twig of the current year, shearing leaves, washing with water, and soaking into laundry detergent; sterilizing with alcohol, then sterilizing with mercury bichloride, and washing with sterile water; cutting a 1cm stem with a bud, and inoculating the stem into the mixture of an inducing culture medium MS, 0.5mg / L 6BA and 0.3mg / L NAA to perform axillary bud inducing culture; selecting a stem with an inducing-germinated sterile axillary bud, and inoculating the stem into the mixture of a proliferation culture medium 3 / 4 MS, 0.4mg / L 6BA and 0.2mg / L NAA to perform cluster bud proliferation culture; cutting a 1cm cluster bud, inoculatinga robust budling with a terminal bud into the mixture of a rooting culture medium 1 / 2 MS and 0.5mg / L ABT, and sequentially performing dark culture and light culture to perform inducing rooting. The Acer fabri cluster bud inducing and rooting technology has the advantages that callus generation is avoided, seed tree merits are kept, variation is avoided, the rooting technology is broken through, and Acer fabri vegetative propagation and industrial detoxification seedling culture are achieved.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to the technique of inducing clustered buds and rooting of the red-winged acer. Background technique [0002] At present, the induction of clustered buds of the red-winged Acer is generally adopted through callus differentiation. This method has the following three defects: First, the existing tissue culture technology of the red-winged Acer is through the callus approach, and the induced clustered buds have a certain degree of inadequacy. Variation, the variation rate is randomly distributed, up to 100%; second, the existing red-winged maple tissue culture technology can not achieve plant regeneration, can not meet the requirements of asexual reproduction; third is the tissue culture technology through the callus approach, its Tissue culture rooting technology has not been reported yet, even if the rooting technology breaks through, the probability of variation of its offspring is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王友生简丽华黄钦府林秀凤梁梅华
Owner 福建省龙岩市林业科学研究所
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