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A molecular marker banii-1 related to the resistance of melon powdery mildew and its application

A melon powdery mildew and molecular marker technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of melon powdery mildew-resistant materials, lack of powdery mildew resistance and unclear distinction of physiological races of powdery mildew. , to achieve the effect of speeding up breeding work efficiency, improving breeding efficiency and easy operation

Active Publication Date: 2022-04-22
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are the following technical problems: First, there are few powdery mildew-resistant melon materials, and the physiological races of powdery mildew are not clearly distinguished
Secondly, most of the molecular markers that have been published so far are difficult to closely combine with related traits, so there are very few molecular markers that can be used for gene mapping and molecular marker-assisted selection of powdery mildew resistance in melon

Method used

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  • A molecular marker banii-1 related to the resistance of melon powdery mildew and its application
  • A molecular marker banii-1 related to the resistance of melon powdery mildew and its application
  • A molecular marker banii-1 related to the resistance of melon powdery mildew and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Screening, primer design and application (verification) of molecular marker BanII-1:

[0030] The F 1 , F 2 The generation population is the experimental material verification marker to identify whether the melon is resistant to powdery mildew.

[0031] Two muskmelon lines M4-125 and X055 and F 2 The high powdery mildew resistant plants and highly susceptible powdery mildew plants in the population were sequenced, and the genome sequence of the melon was obtained through the NCBI website. Through the comparison of the sequence and the sequencing material, the chromosome of the sequence of the segment of the melon powdery mildew resistance gene was obtained. Interval, within this interval, mining the base segment of the sequencing data with the SNP mutation site, analyzing the enzyme digestion information of the site, obtaining the sequence with the CAPS mutation, and using the Primer software to design the molecular marker BanII on the sequence with the site...

Embodiment 2

[0049] Example 2 Expanded range application (verification) of molecular marker BanII-1:

[0050] Using the obtained molecular marker BanII-1 to screen the germplasm resources of melon varieties with different resistance to powdery mildew (physiological race 2F of Erysipha monocysticum):

[0051] In order to identify the applicability of the developed molecular marker BanII-1 to other melon materials, 10 kinds of melon materials with different resistance to powdery mildew (Erysipha monocysticum race 2F) including M4-125 and X055 were selected. Using the molecular marker primers, the genome was amplified by PCR and verified by enzyme digestion. The names and resistances of these 10 kinds of melon materials are shown in Table 2

[0052] Table 2 Introduction of 10 kinds of melon materials

[0053]

[0054] Expanded range of applications of the molecular marker BanII-1:

[0055] (1) Collect the young true leaves of the above 10 kinds of melon materials, and use the improved C...

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Abstract

The invention discloses a molecular marker BanII‑1 related to the resistance of melon powdery mildew and its application, and relates to a biomolecular marker and its application. The application comprises the following steps: (1) extracting the melon genome to be tested; (2) extracting the melon genome to be tested; The genome is used as a template, and molecular marker primers are used for PCR amplification to obtain PCR products; (3) enzyme digestion is performed on the obtained PCR products to obtain enzyme digestion products; (4) the obtained enzyme digestion products are subjected to agarose gel electrophoresis For identification, the melon plant to be tested is judged to be melon resistant to E. monocystica 2F physiological race or the melon not resistant to E. monocysticum 2F physiological race according to the fragment size of the enzyme-digested product obtained from the identification. The provided molecular markers are universal in different materials, and the operation is simple and the results are clear. Through the method of molecular marker-assisted selection, the resistance to powdery mildew of melon can be assisted selection at the seedling stage of melon, which greatly improves the breeding efficiency and shortens the breeding period.

Description

technical field [0001] The invention relates to a molecular marker and its application, in particular to a molecular marker related to the powdery mildew resistance of melon and its application. Background technique [0002] Molecular marker-assisted selection (MAS) is a fast and simple method for screening target traits. It can be done in the off-season without environmental constraints, allowing simultaneous screening for several traits. In MAS, molecular markers that are tightly linked to traits of interest are localized in plants. The gene segment of interest is then introduced into the donor plant by crossing, and during backcrossing, molecular markers are used to anchor the gene of interest until the gene of interest is stably inherited in the final plant variety. [0003] Powdery mildew is one of the main fungal diseases affecting the yield and quality of muskmelon. It occurs widely in open field cultivation and protected cultivation of muskmelon. The powdery mildew...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 高鹏崔浩楠栾非时刘识丁卓王学征
Owner NORTHEAST AGRICULTURAL UNIVERSITY