Joint separation method for lysozyme and ovotransferrin in egg white

A technology of ovotransferrin and lysozyme, applied in the field of protein separation and purification, can solve the problems of low separation efficiency, difficult industrialization and the like, and achieve the effects of simple operation, low cost and efficient separation

Active Publication Date: 2019-07-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current separation methods for lysozyme and ovotransferrin generally have the problems of low separation efficiency and difficulty in indu...

Method used

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  • Joint separation method for lysozyme and ovotransferrin in egg white
  • Joint separation method for lysozyme and ovotransferrin in egg white
  • Joint separation method for lysozyme and ovotransferrin in egg white

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Egg liquid pretreatment: Stir the egg white liquid and adjust the pH to 8;

[0047] (2) Adsorption by metal chelating cation resin: mix carboxyl type weak cation exchange resin and pretreated egg white liquid according to the ratio of material to liquid 1:5, add ferric chloride, so that the molar concentration of ferric chloride in egg white liquid is 0.2mmol / L, adsorbed for 5 hours, and removed the supernatant A after the adsorption was completed;

[0048] (3) Cleaning: add NaCl solution with a concentration of 0.9% to the adsorbed resin according to the ratio of material to liquid 1:3, remove the supernatant after shaking and mixing, and repeat cleaning 5 times;

[0049] (4) Elution: Add NaCl solution with a concentration of 5% to the cleaned resin according to the ratio of solid to liquid at 1:3, collect the clear liquid after shaking and eluting for 30 minutes, repeat the operation 5 times, collect all the eluents and mix them evenly to obtain Eluent A;

[00...

Embodiment 2

[0058] (1) Egg liquid pretreatment: Stir the egg white liquid and adjust the pH to 9.5;

[0059] (2) Adsorption by metal chelating cation resin: mix carboxyl-type weak cation exchange resin and pretreated egg white liquid according to the ratio of material to liquid 1:5, add ferric chloride, so that the molar concentration of ferric chloride in the egg white liquid is 0.1mmol / L, adsorbed for 5 hours, and removed the supernatant A after the adsorption was completed;

[0060] (3) Cleaning: add a NaCl solution with a concentration of 0.9% to the adsorbed resin according to the ratio of material to liquid 1:3, remove the supernatant after shaking and mixing, and repeat the cleaning 4 times;

[0061] (4) Elution: Add NaCl solution with a concentration of 7% to the cleaned resin according to the ratio of solid to liquid at 1:3, collect the clear liquid after shaking and eluting for 30 minutes, repeat the operation 4 times, collect all the eluents and mix them evenly to obtain Elue...

Embodiment 3

[0070] (1) Egg liquid pretreatment: Stir the egg white liquid and adjust the pH to 6.5;

[0071] (2) Adsorption by metal chelating cation resin: mix carboxyl type weak cation exchange resin and pretreated egg white liquid according to the ratio of material to liquid 1:5, add ferric chloride, so that the molar concentration of ferric chloride in egg white liquid is 0.2mmol / L, adsorbed for 5 hours, and removed the supernatant A after the adsorption was completed;

[0072] (3) Cleaning: add NaCl solution with a concentration of 0.9% to the adsorbed resin according to the ratio of material to liquid 1:3, remove the supernatant after shaking and mixing, and repeat cleaning 5 times;

[0073] (4) Elution: Add NaCl solution with a concentration of 5% to the cleaned resin according to the ratio of solid to liquid at 1:3, collect the clear liquid after shaking and eluting for 30 minutes, repeat the operation 5 times, collect all the eluents and mix them evenly to obtain Eluent A;

[...

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Abstract

The invention discloses a joint separation method for lysozyme and ovotransferrin in egg white, and belongs to the technical field of protein separation and purification. The method comprises the steps that (1) the pH of egg white liquid is adjusted to 6.5-9.5, and metal-chelating cationic resin is adopted for adsorbing the lysozyme and the ovotransferrin; (2) eluant is subjected to crystallization treatment to obtain lysozyme crystals; (3) supernate after crystallization is collected, anion exchange resin is adopted for adsorption, the eluant is subjected to ultrafiltration and drying, and the ovotransferrin is obtained. The method can efficiently separate the lysozyme and the ovotransferrin from the egg white at the same time, the yield reaches 75% or above, the purity can reach 95% or above, and the adopted method has the advantages that the operation is simple, and the cost is low and is easily applied to industrialization.

Description

technical field [0001] The invention relates to a combined separation method of lysozyme and ovotransferrin in egg white, belonging to the technical field of protein separation and purification. Background technique [0002] Egg white protein is the most ideal high-quality protein in food, mainly composed of ovalbumin, ovotransferrin, ovomucin and lysozyme. Among them, lysozyme and ovotransferrin are biologically active proteins that have attracted the attention of researchers and have great application potential. [0003] Lysozyme is an essential antibacterial protein for the growth and development of infants. It can not only kill intestinal putrefaction cocci, but also enhance the immune function of immunoglobulins and improve the infant's anti-infection ability, especially for premature infants to prevent weight loss, It has the effects of preventing digestive organ diseases and increasing body weight, and is a good auxiliary material for infant functional foods. [000...

Claims

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Application Information

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IPC IPC(8): C12N9/36C07K14/79C07K1/18
CPCC07K14/79C07K2299/00C12N9/2462C12Y302/01017
Inventor 杨严俊苏宇杰常翠华李俊华顾璐萍
Owner JIANGNAN UNIV
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