Joint separation method for lysozyme and ovotransferrin in egg white
A technology of ovotransferrin and lysozyme, applied in the field of protein separation and purification, can solve the problems of low separation efficiency, difficult industrialization and the like, and achieve the effects of simple operation, low cost and efficient separation
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Embodiment 1
[0046] (1) Egg liquid pretreatment: Stir the egg white liquid and adjust the pH to 8;
[0047] (2) Adsorption by metal chelating cation resin: mix carboxyl type weak cation exchange resin and pretreated egg white liquid according to the ratio of material to liquid 1:5, add ferric chloride, so that the molar concentration of ferric chloride in egg white liquid is 0.2mmol / L, adsorbed for 5 hours, and removed the supernatant A after the adsorption was completed;
[0048] (3) Cleaning: add NaCl solution with a concentration of 0.9% to the adsorbed resin according to the ratio of material to liquid 1:3, remove the supernatant after shaking and mixing, and repeat cleaning 5 times;
[0049] (4) Elution: Add NaCl solution with a concentration of 5% to the cleaned resin according to the ratio of solid to liquid at 1:3, collect the clear liquid after shaking and eluting for 30 minutes, repeat the operation 5 times, collect all the eluents and mix them evenly to obtain Eluent A;
[00...
Embodiment 2
[0058] (1) Egg liquid pretreatment: Stir the egg white liquid and adjust the pH to 9.5;
[0059] (2) Adsorption by metal chelating cation resin: mix carboxyl-type weak cation exchange resin and pretreated egg white liquid according to the ratio of material to liquid 1:5, add ferric chloride, so that the molar concentration of ferric chloride in the egg white liquid is 0.1mmol / L, adsorbed for 5 hours, and removed the supernatant A after the adsorption was completed;
[0060] (3) Cleaning: add a NaCl solution with a concentration of 0.9% to the adsorbed resin according to the ratio of material to liquid 1:3, remove the supernatant after shaking and mixing, and repeat the cleaning 4 times;
[0061] (4) Elution: Add NaCl solution with a concentration of 7% to the cleaned resin according to the ratio of solid to liquid at 1:3, collect the clear liquid after shaking and eluting for 30 minutes, repeat the operation 4 times, collect all the eluents and mix them evenly to obtain Elue...
Embodiment 3
[0070] (1) Egg liquid pretreatment: Stir the egg white liquid and adjust the pH to 6.5;
[0071] (2) Adsorption by metal chelating cation resin: mix carboxyl type weak cation exchange resin and pretreated egg white liquid according to the ratio of material to liquid 1:5, add ferric chloride, so that the molar concentration of ferric chloride in egg white liquid is 0.2mmol / L, adsorbed for 5 hours, and removed the supernatant A after the adsorption was completed;
[0072] (3) Cleaning: add NaCl solution with a concentration of 0.9% to the adsorbed resin according to the ratio of material to liquid 1:3, remove the supernatant after shaking and mixing, and repeat cleaning 5 times;
[0073] (4) Elution: Add NaCl solution with a concentration of 5% to the cleaned resin according to the ratio of solid to liquid at 1:3, collect the clear liquid after shaking and eluting for 30 minutes, repeat the operation 5 times, collect all the eluents and mix them evenly to obtain Eluent A;
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