Molecular marker for cancer diagnosis

A technology of gastric adenocarcinoma and reagents, applied in the field of molecular markers for cancer diagnosis, can solve problems such as unclear master-slave relationship of lncRNA and unclear role of 1ncRNA

Inactive Publication Date: 2019-07-30
XUZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the specific role and master-slave relationship of lncRNA in the occurrence and development of gastric cancer is still unclear, and the role of many gastric cancer-related lncRNAs is unknown

Method used

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  • Molecular marker for cancer diagnosis
  • Molecular marker for cancer diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Screening for gene markers associated with gastric adenocarcinoma

[0037] 1. Sample collection

[0038] The cancer tissues and corresponding paracancerous tissue samples of 4 cases of gastric adenocarcinoma were collected for high-throughput sequencing. All patients did not receive chemotherapy, radiotherapy and endocrine therapy before operation.

[0039] 2. RNA sample preparation and quality analysis

[0040] Total RNA was extracted using Tiangen Animal Tissue Total RNA Extraction Kit (catalogue number: DP431), and the steps are detailed in the instructions.

[0041] 1) Homogenization treatment

[0042] Add 300μl Lysis Solution RL for every 10-20mg tissue, and grind the tissue thoroughly with a grinding pestle; then add 590μl RNase-Free ddH to the homogenate 2 O and 10 μl Proteinase K, mix well and treat at 56°C for 10-20min.

[0043] 2) Centrifuge at 12,000rpm for 2-5min, and take the supernatant for the following operations.

[0044] 3) Slowly add 0....

Embodiment 2

[0076] Example 2QPCR sequencing verification of differential expression of RP11-576I22.2 gene

[0077] 1. The differential expression of the RP11-576I22.2 gene was verified by large-scale QPCR on the cancer tissue samples and para-cancerous tissue samples collected from 31 gastric adenocarcinoma patients according to the collection method in Example 1.

[0078] 2. RNA extraction

[0079] The total RNA was extracted using Tiangen Animal Tissue Total RNA Extraction Kit (Cat. No. DP431). See Example 1 for specific steps.

[0080] 3. QPCR

[0081] Primers were designed according to the gene sequences of RP11-576I22.2 and GADPH, and the primer sequences were as follows:

[0082] RP11-576I22.2:

[0083] Forward primer: 5′-AGTATGAGGTAGCCAACA-3′ (SEQ ID NO.1)

[0084] Reverse primer: 5'-AACTTGACTTCTGACATCTT-3' (SEQ ID NO.2)

[0085] GAPDH:

[0086] Forward primer: 5'-AATCCCATCACCATCTTCCAG-3' (SEQ ID NO.3)

[0087] Reverse primer: 5'-GAGCCCCAGCCTTTCTCAT-3' (SEQ ID NO.4)

[0088...

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Abstract

The invention discloses a molecular marker for cancer diagnosis. Through a high-throughput sequencing technology, firstly, up-regulated expression of RP11-576I22.2 in gastric adenocarcinoma is found,and that is to say, whether or not a testee suffers from gastric adenocarcinoma can be judged by detecting the expression level of RP11-576I22.2.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a molecular marker for cancer diagnosis, the molecular marker is RP11-576I22.2. Background technique [0002] Gastric cancer is one of the most common malignant tumors, its global incidence ranks fourth among all malignant tumors, and its case fatality rate ranks second. There are nearly one million new cases of gastric cancer worldwide every year, and nearly 50.0% of them occur in China. In my country, there were 679,000 new cases of gastric cancer in recent years, ranking second among all tumors, and 498,000 deaths from gastric cancer each year, ranking third only among liver cancers (W Chen, R Zheng, PD Baade, et al. Cancer statistics in China, 2015. CA Cancer J Clin 2016.). The most effective treatment for gastric cancer is radical surgery. However, the diagnosis and treatment rate of early gastric cancer in my country is low, and most patients are often in the advanced stage when th...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 董东郑骏年李曼
Owner XUZHOU MEDICAL UNIV
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