Gene for regulating and controlling corn flowering period, promoter, and application of gene and promoter

A flowering period and promoter technology, applied in the application field of regulating corn flowering period genes and promoters, regulating corn flowering period or the number of leaves, can solve the problem of maize flowering period without SPL gene

Active Publication Date: 2019-08-02
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no report on the phenotypes of maize flowering period and leaf number regulated by SPL gene.

Method used

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  • Gene for regulating and controlling corn flowering period, promoter, and application of gene and promoter
  • Gene for regulating and controlling corn flowering period, promoter, and application of gene and promoter
  • Gene for regulating and controlling corn flowering period, promoter, and application of gene and promoter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 The variation site in the ZmSBP29 promoter region regulates the flowering period and leaf number of maize by controlling the gene expression of ZmSBP29

[0053] 1. Discovery of variant sites in the promoter region of ZmSBP29

[0054] Using the deep (>10×) resequencing data of 350 maize inbred lines combined with the published maize B73 V3 genome, 25,320,664 single nucleotide polymorphism molecular markers (SNPs) and 4,319,510 indel polymorphisms were mined Sex molecular markers (Indel). The population structure and genetic relationship of 350 maize inbred lines were estimated using these mined molecular markers, and then the genome-wide association analysis (GWAS) was performed by combining the phenotypes of flowering stage and leaf number collected from 4 environments. Among them, two SNPs Chr7_151256322_G / C, Chr7_151256317_C / T, and one Indel Indel_7_151256304_5 / 0 on chromosome 7 were found to be significantly associated with the flowering period and leaf nu...

Embodiment 2

[0059] Example 2 Creation and phenotypic analysis of ZmSBP29 overexpression transgenic event SBP29-OE1

[0060] 1. Construction and genetic transformation of ZmSBP29 gene overexpression vector

[0061] The CDS sequence of the ZmSBP29 gene in the B73 genome was cloned by PCR, and the PCR product was inserted between the BamH I and Pst I restriction sites of the plant overexpression vector CPB-UBI-EGFP with the In-Fusion Advantage Kit ( Figure 4 ); after the final constructed gene overexpression vector was verified to be correct by PCR sequencing, the maize inbred line ZC01 was transformed by the method mediated by Agrobacterium.

[0062] 2. Acquisition of ZmSBP29 overexpression transgenic event SBP29-OE1

[0063] The T0 generation transgenic seeds obtained in step 1 were planted in the Langfang Transgenic Base, Hebei Province in the summer of 2017, and 5 transgenic events were sampled at the V5 stage, quick-frozen in liquid nitrogen, and then the RNA was extracted by the TRIz...

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PUM

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Abstract

The invention discloses a gene for regulating and controlling the corn flowering period, a promoter, and application of the gene and the promoter. Firstly, a key genome region for regulating and controlling the corn flowering period or the leaf quantity is provided; the polyribonucleotide sequence is shown as SEQ ID No. 1; the condition that a ZmSBP29 gene has the function of regulating and controlling the corn flowering period and the leaf quantity is proved by a genetics and molecular biology method. The invention further provides a key genome variation for regulating and controlling the expression quantity of the ZmSBP29 gene, and creates a transgenic event for overexpression of the ZmSBP29 gene; the key genome variation and the transgenic event can be applied to the improvement of thecorn flowering period and plant type, and can also be applied to corn high yield and adaptive breeding.

Description

technical field [0001] The present invention relates to genes and promoters regulating the flowering stage of maize, in particular to promoter mutants and detection primers for regulating the ZmSBP29 gene. The present invention further relates to their application in regulating the flowering stage or leaf quantity of maize, which belongs to the flowering stage and leaves of maize Quantity in the field of gene regulation. Background technique [0002] Corn is an important crop integrating grain, feed, and industrial raw materials. It is currently one of the most widely planted and most demanded cereal crops in China and the world. Appropriate flowering period is one of the key factors to determine the adaptability of maize to planting in different ecological environments, and then to ensure its production supply. There are 6 major corn planting areas in China, among which, the northern spring corn area and the Huanghuaihai plain summer corn area are the two major corn produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/113C12N15/82C12N15/11C12N15/65A01H5/02A01H5/12A01H6/46
CPCC07K14/415C12N15/65C12N15/8261C12N15/827C12N2800/22
Inventor 王海洋王宝宝魏洪彬赵永平孔德鑫谢钰容
Owner SOUTH CHINA AGRI UNIV
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