Preparation method and application of humanized gene edited mammal

Abstract

The invention relates to a gene humanized mammal and application of the gene humanized mammal. The gene humanized mammal expresses human serum albumin. In certain embodiments, a genome of the humanized gene edited mammal comprises polyribonucleotide for encoding the human serum albumin, and can comprise modification on human serum albumin genes.

Description

technical field [0001] The present invention relates to humanized gene editing technology, in particular to a preparation method and application of a humanized gene edited mammal expressing human serum albumin. Background technique [0002] Human serum albumin (Human serum albumin, HSA) is a single-chain, non-glycosylated protein synthesized by the liver. It plays many important roles in the body, such as maintaining plasma colloid osmotic pressure, nutrient transport, anticoagulation, and scavenging free radicals. In clinical practice, HSA has multiple uses such as treating burns, shock caused by blood loss, preventing hypoalbuminemia, edema or ascites caused by liver cirrhosis or kidney disease, etc. In addition, HSA can also be used as a medium component of many host cells and engineering cell lines and Stabilizers for biological products. The source of HSA mainly depends on human raw material plasma. In recent years, genetic engineering has provided an attractive solut...

AI Technical Summary

Problems solved by technology

Although many laboratories and companies around the world have conducted extensive research on the production of recombinant HSA (rHSA) from bacteria, yeast, plants, and mammary glands of transgenic animals, so far few have been cost-effective for large-scale production

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