Method for detecting cancer marker by using manganese dioxide modified upconversion nanometer material

A technology of manganese dioxide and nanomaterials, which is applied in the direction of material excitation analysis, preparation of test samples, fluorescence/phosphorescence, etc., can solve the problems of poor biocompatibility, low sensitivity, and poor selectivity, and achieve low cost and high selectivity. High, low detection limit effect

Active Publication Date: 2019-08-02
TIANJIN NORMAL UNIVERSITY
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  • Application Information

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Problems solved by technology

[0005] The currently reported methods for detecting cancer markers mainly include colorimetric methods, fluorescence methods, chromatographic methods and electrochemical methods. Therefore, it is necessary to develop a method for detecting alkaline phosphatase using manganese dioxide-modified upconversion nanomaterials with simple operation, rapid response and high sensitivity.

Method used

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  • Method for detecting cancer marker by using manganese dioxide modified upconversion nanometer material
  • Method for detecting cancer marker by using manganese dioxide modified upconversion nanometer material
  • Method for detecting cancer marker by using manganese dioxide modified upconversion nanometer material

Examples

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Embodiment 1

[0046] Ytterbium and thulium doped sodium yttrium tetrafluoride (NaYF 4 :Yb, Tm) Synthesis of upconversion nanoparticles:

[0047] (1) Pre-treatment nitrification: weigh Y 2 o 3 , Yb 2 o 3 , Tm 2 o 3 Three rare earth oxides, the molar ratio of which is 75:25:0.3, was added to beaker A, and then 2 mL of concentrated nitric acid and 2 mL of high-purity water were added in sequence, and the small beaker was placed in an electric heating mantle to heat until the oxides were nitrated completely. Continue to add 5 mL of high-purity water after nitrate formation, and continue to add 5 mL of high-purity water when it is about to evaporate to dryness, measure the pH value of the solution at this time, add high-purity water repeatedly until the pH value of the solution is about 7, evaporate to dryness, add 5 mL of high-purity water for later use .

[0048] (2) Synthesis: Weigh 10 mmol each of sodium hydroxide and ammonium fluoride into beaker B, and continue to weigh 0.15 g of po...

Embodiment 2

[0051] Ytterbium and thulium doped sodium yttrium tetrafluoride (NaYF 4 :Yb, Tm ) synthesis of upconversion nanoparticles:

[0052] (1) Pre-treatment nitrification: weigh Y 2 o 3 , Yb 2 o 3 , Tm 2 o 3 Three rare earth oxides, the molar ratio of which is 75:25:0.3, was added to beaker A, and then 2 mL of concentrated nitric acid and 2 mL of high-purity water were added in sequence, and the small beaker was placed in an electric heating mantle to heat until the oxides were nitrated completely. Continue to add 5 mL of high-purity water after nitrate formation, and continue to add 5 mL of high-purity water when it is about to evaporate to dryness, measure the pH value of the solution at this time, add high-purity water repeatedly until the pH value of the solution is about 7, evaporate to dryness, add 5 mL of high-purity water for later use .

[0053] (2) Synthesis: Weigh 10 mmol each of sodium hydroxide and ammonium fluoride into beaker B, and continue to weigh 0.15 g of p...

Embodiment 3

[0056] Ytterbium and thulium doped sodium yttrium tetrafluoride (NaYF 4 :Yb, Tm) Synthesis of upconversion nanoparticles:

[0057] (1) Pre-treatment nitrification: weigh Y 2 o 3 , Yb 2 o 3 , Tm 2 o 3 Three rare earth oxides, the molar ratio of which is 75:25:0.3, was added to beaker A, and then 2 mL of concentrated nitric acid and 2 mL of high-purity water were added in sequence, and the small beaker was placed in an electric heating mantle to heat until the oxides were nitrated completely. Continue to add 5 mL of high-purity water after nitrate formation, and continue to add 5 mL of high-purity water when it is about to evaporate to dryness, measure the pH value of the solution at this time, add high-purity water repeatedly until the pH value of the solution is about 7, evaporate to dryness, add 5 mL of high-purity water for later use .

[0058] (2) Synthesis: Weigh 10 mmol each of sodium hydroxide and ammonium fluoride into beaker B, and continue to weigh 0.15 g of po...

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Abstract

The invention discloses a method for detecting alkaline phosphatase by using manganese dioxide modified upconversion nanoparticles. An upconversion nanoparticle size of ytterbium and thulium-doped tetrafluorohydrazine sodium yttrium (NaYF4: Yb, Tm) is about 2nm and an emission spectrum is 471nm. A synthetic method of the upconversion nanoparticles of the ytterbium and thulium-doped tetrafluorohydrazine sodium yttrium (NaYF4: Yb, Tm) and the upconversion nanoparticles of manganese dioxide modification is simple. Operation for detecting the alkaline phosphatase is simple, an expensive apparatusis not needed and the operation is fast and is sensitive. During biological sample detection, an interference of autofluorescence can be avoided and toxicity is small. A linear range of the alkaline phosphatase is 0.1 U / L-100 U / L, and a detection limit is 0.41 U / L. The method has an excellent application prospect in actual biological sample analysis detection and bio-imaging.

Description

[0001] This patent is supported by the General Project of the National Natural Science Foundation of China (21375095) and the Youth Project of the Tianjin Natural Science Foundation of China (No.17JCQNJC05800). technical field [0002] The invention belongs to the technical field of biological analysis and detection, and relates to the detection application of a lamellar amorphous manganese dioxide-modified up-conversion nanomaterial to cancer marker alkaline phosphatase. Background technique [0003] Up-conversion nanomaterials can usually convert two or more low-energy photons into high-energy photons for emission, because the luminescence of up-conversion materials is to absorb low-energy light with a long wavelength (usually 980 nm near-infrared light) and emit High-energy light with a short wavelength is also called anti-Stokes luminescence. The upconversion process of rare earth doping mainly depends on the ladder-like energy level of rare earth ions, and the 4f electr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/38
CPCG01N21/64G01N1/38
Inventor 李妍梁美玉张菲霍建中陈文欣王璐
Owner TIANJIN NORMAL UNIVERSITY
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