Method and kit for detecting exogenous N-Methylpyrrole-2-carboxaldehyde

A pyrrole formaldehyde, exogenous technology, applied in the field of analytical chemistry, can solve the problems of difficult to distinguish the source of spice compounds, the method needs to be improved, etc., and achieve the effect of maintaining legal rights, low cost and simple operation

Inactive Publication Date: 2019-08-02
CHINESE ACAD OF INSPECTION & QUARANTINE
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When banned flavor ingredients are detected in food, it is difficult to distinguish the source of flavor compounds based on GC-MS/MS

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and kit for detecting exogenous N-Methylpyrrole-2-carboxaldehyde
  • Method and kit for detecting exogenous N-Methylpyrrole-2-carboxaldehyde
  • Method and kit for detecting exogenous N-Methylpyrrole-2-carboxaldehyde

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] In this embodiment, the endogenous and industrially synthesized N-methyl-2-pyrrole formaldehyde δ in natural coffee beans were measured respectively 13 C value, as follows:

[0061] (1) Extraction treatment: (a) A coffee bean sample without any added exogenous N-methyl-2-pyrrole formaldehyde was pulverized.

[0062] (b) Weigh 200g of sample, place it in a 1L flat-bottomed flask, add extraction reagent B: ultrapure water, connect to one side of the Likens-Nickerson simultaneous distillation extraction device, add extraction reagent A: dichloromethane to a 250mL flat-bottomed flask In the middle, it is connected to the other side of the Likens-Nickerson simultaneous distillation extraction device, and the flat-bottomed flasks on both sides are heated by an electric heating plate.

[0063] (c) After heating and extracting for 180 minutes, the extract was dried with a desiccant. Then, the extract was concentrated to 1 mL using a rotary evaporator set at 35 °C.

[0064] T...

Embodiment 2

[0072] The method for detecting exogenous N-methyl-2-pyrrole formaldehyde in the embodiment of the present invention is used to detect the exogenous N-methyl-2-pyrrole formaldehyde in coffee samples, specifically as follows:

[0073] (1) Extraction processing:

[0074] (a) Weigh 200g of coffee sample, place it in a 1L flat-bottomed flask, add extraction reagent B, and connect it to one side of the Likens-Nickerson simultaneous distillation extraction device.

[0075] (b) Add Extraction Reagent A to a 250ml flat bottom flask, connected to the other side of the Likens-Nickerson Simultaneous Distillation Extraction Unit. The flat-bottomed flasks on both sides are heated by an electric heating plate.

[0076] (c) After heating and extracting for 180 minutes, the extract was dried with a desiccant. Then, the extract was concentrated to 1 mL using a rotary evaporator set at 35 °C.

[0077] (2) According to the method of embodiment 1, the extract is carried out to gas chromatograp...

Embodiment 3

[0083] In this embodiment, different concentrations of N-methyl-2-pyrrole formaldehyde are carried out δ 13 C determination, specifically as follows:

[0084] 1) The N-methyl-2-pyrrole formaldehyde standard substance (1-50ppm) of different concentrations was separated by gas chromatography according to the method of Example 1, and then the carbon isotope ratio was measured.

[0085] 2) The response results of the N-methyl-2-pyrrole formaldehyde standard substance of different concentrations measured are as follows figure 1 As shown, the results show that the target is completely oxidized to CO 2 , the recovery rate is good, indicating that different concentrations of N-methyl-2-pyrrole formaldehyde extracted from food samples can achieve accurate determination of carbon isotope ratio.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method and a kit for detecting exogenous N-Methylpyrrole-2-carboxaldehyde, wherein the method for detecting the exogenous N-Methylpyrrole-2-carboxaldehyde comprises the following steps: extracting a sample to obtain an extracting solution; separating the extracting solution by gas chromatography to obtain separated N-Methylpyrrole-2-carboxaldehyde; detecting an isotope ratio of the separated N-Methylpyrrole-2-carboxaldehyde so as to obtain a detection result of the isotope ratio; and determining whether the sample contains exogenous N-Methylpyrrole-2-carboxaldehyde based on the detection result of the isotope ratio. The method can judge whether a sample contains exogenous N-Methylpyrrole-2-carboxaldehyde.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method and a kit for detecting exogenous N-methyl-2-pyrrole formaldehyde. Background technique [0002] Flavor has the characteristics of enhancing the existing flavor of food and imparting special flavor to food, and is widely used in the food industry. Considering the health risks posed by some fragrance ingredients, international restrictions have been placed on the fragrance compounds that can be used. my country's national food safety standard GB2760-2014 "Usage Standards for Food Additives" clearly limits the ingredients of flavors and fragrances that can be added to food, especially stipulates that no flavors and fragrances can be added to tea, coffee and pasteurized milk. [0003] Although gas chromatography, liquid chromatography, gas chromatography-tandem mass spectrometry and other technologies commonly used in laboratories can determine the types and contents of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884G01N2030/8868
Inventor 张峰刘通王秀娟王玉娇
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap