Application of Chinese pulsatilla root extract product in preparation of drugs for treating viral and/or bacterial diseases

A technology of Pulsatilla saponins and medicines, which can be used in sexual diseases, antibacterial drugs, plant/algae/fungus/moss components, etc., can solve unseen problems, achieve strong biological activity, reduce economic losses, and excellent therapeutic effects.

Active Publication Date: 2019-08-06
广西英路维特药物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no report about the compounds of the present invention being used for the treatment of viral and / or bacterial mastitis in cows

Method used

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  • Application of Chinese pulsatilla root extract product in preparation of drugs for treating viral and/or bacterial diseases
  • Application of Chinese pulsatilla root extract product in preparation of drugs for treating viral and/or bacterial diseases
  • Application of Chinese pulsatilla root extract product in preparation of drugs for treating viral and/or bacterial diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, the preparation of compound of the present invention

[0029] Take 100 kg of Pulsatillae medicinal material, add 10 times the amount of 70% ethanol to heat and reflux for extraction twice, concentrate the extract under reduced pressure at 75°C, centrifuge at 4000r / min for 10min, pass the supernatant through a polar macroporous adsorption resin column, and then use water and 30% ethanol in sequence , 70% ethanol elution, after getting 70% ethanol elution part 75 ℃ of decompression concentration, spray-drying obtains Pulsatilla grandis total saponin extract 3450g; 10 μm ODS), using 50% methanol for elution, collecting the corresponding eluent according to the chromatographic peak, concentrating under reduced pressure, and freeze-drying to obtain Pulsatilla saponin B4 1700g.

Embodiment 2

[0030] Embodiment 2, the assay of compound of the present invention

[0031] 1. Instruments and reagents

[0032] Agilent 1260 high performance liquid chromatography and DAD ultraviolet detector were purchased from Agilent Technologies (China) Co., Ltd., BP211D electronic analytical balance was purchased from German Sartorius company, and KQ-400DB numerical control ultrasonic cleaner was purchased from Kunshan Ultrasonic Instrument Co., Ltd. .

[0033] Pulsatilla saponin B4 sample, prepared in Example 1.

[0034] Pulsatilla saponin B4 reference substance (batch number: 111766-201702, content: 94.7%) was purchased from China National Institutes for Food and Drug Control.

[0035] 2. Chromatographic conditions

[0036] Chromatographic column: Sepax Bio-C18 (4.6×250mm, 5μm); mobile phase: methanol-water (64:36); detection wavelength is 201nm; flow rate: 1.0mL / min.

[0037] 3. Preparation of reference solution

[0038] Take an appropriate amount of Pulsatillae saponin B4 refe...

Embodiment 3

[0055] Embodiment 3, the preparation of Pulsatilla saponin B4 injection of the present invention

[0056] 1) Prescription: 50g Pulsatilla saponin B4 is added with water to prepare 1000mL injection.

[0057] 2) Preparation method: Weigh 50 g of Pulsatilla saponin B4 prepared in Example 1, add 800 mL of water for injection, dissolve it completely, filter, adjust the pH to 7.0, add water for injection to 1000 mL, fine filter, fill, seal, 100 °C Sterilize for 30 minutes, that is.

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Abstract

The invention discloses an application of a compound pulchinenoside B4 shown as a formula I or pharmaceutically acceptable salt thereof in the preparation of drugs for treating viral and / or bacterialdiseases. The pulchinenoside B4 is a natural effective component extracted and separated from a traditional Chinese medicinal material Chinese pulsatilla root, and is non-toxic and non-drug resistant.The compound pulchinenoside B4 has strong biological activity and excellent treating effects on cow mastitis, and the abandonment of milk is not needed during treatment and at the end of treatment, so that the economic loss of farmers can be reduced, and risks of drug resistance generation can be reduced as well. In addition, the pulchinenoside B4 has excellent treating effects on clinic mastitisand also on long-term cure and recessive mastitis. A pulchinenoside injection has the characteristics of being safe and efficient and has no residues, no drug resistance and no toxic side effects when being applied to the treatment of cow mastitis.

Description

technical field [0001] The invention relates to the use of Pulsatilla pulsatillae extract in the preparation of medicines for treating viral and / or bacterial diseases. Background technique [0002] Pulsatilla Chinensis (Bge.) Regel is a plant of the genus Pulsatilla (Ranunculaceae), and its main medicinal part is its dry root. The earliest record about Pulsatilla is "Shen Nong's Materia Medica". It tastes bitter, cool in nature, and returns to the stomach, kidney, and large intestine. It also has a good therapeutic effect on cold, heat and fever, red eyes and sore eyes. Pulsatilla has a variety of pharmacological activities, mainly improving the body’s ability to recognize and resist the invasion of foreign bodies, reducing the reproduction rate of tumor cells, reducing the number of pathogenic microorganisms, and effectively inhibiting the oxidation of free radicals. Among them, the most potential application is in the anti- Inflammatory anti-tumor new drug development. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P31/04A61P31/12A61P15/14
CPCA61K31/704A61P31/04A61P31/12A61P15/14A61K36/71A61K31/35Y02A50/30A61P31/00A61K9/0019
Inventor 杨世林苏柘僮
Owner 广西英路维特药物有限公司
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