Pharmaceutical composition and applications thereof

A technology of composition and use, applied in the preparation of medicines, in the field of interleukin-6 modulators, can solve the problems of difficult acquisition and unstable properties, and achieve the effects of stable storage, convenient storage, significant application prospects and economic value

Active Publication Date: 2019-08-06
吉林海颐康达生物科技发展有限公司
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, polypeptide (protein) drugs often have the disadvantages of unstable properties and difficulty in obtaining

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pharmaceutical composition and applications thereof
  • Pharmaceutical composition and applications thereof
  • Pharmaceutical composition and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation of embodiment 1 composition of the present invention

[0022] Ginsenoside Rk1, ginsenoside Rg5, and ginsenoside CK were purchased from Xi'an Baile Biotechnology Development Co., Ltd., Chengdu Master Biotechnology Co., Ltd. and extracted from red ginseng, and their purity met the medicinal standard. The composition of the present invention is prepared according to the following specific gravity:

[0023]

Embodiment 2

[0024] The safety evaluation of embodiment 2 compositions of the present invention

[0025] The study of non-clinical safety evaluation of the composition of the present invention is as follows. In the following experiments, the solution of the composition of the present invention prepared in Example 1 was added to the cell culture medium.

[0026] 1. Oral acute toxicity test in mice

[0027] Under the conditions of maximum dosage concentration and maximum dosage volume, mice were orally administered the composition prepared in Example 1, and observed continuously for 14 days to obtain the maximum tolerated dose of the composition of the present invention.

[0028] 2. Beagle dog oral acute toxicity test

[0029] Under the conditions of maximum dosage concentration and maximum dosage volume, the composition prepared in Example 1 was orally administered to Beagle dogs by gavage, so as to obtain the maximum tolerated dose of the composition of the present invention.

[0030] 3...

Embodiment 3

[0038] Embodiment 3 Pharmacodynamics

[0039] 1. The composition of the present invention inhibits the synthesis of interleukin-6 in cells

[0040] Materials: human umbilical vein endothelial cells HUVEC, human embryonic kidney epithelial cells HEK-293, and the formulated composition of the present invention. By accurately weighing 1.5 mg of the composition prepared in Example 1, and dissolving it in 200 μL of 75% ethanol solution to a final concentration of 7.5 mg / ml, the ethanol solution of the composition of Example 1 of the present invention was obtained.

[0041] By accurately weighing 1.5 mg of ginsenoside Rk1, ginsenoside Rg5, ginsenoside CK and total ginsenosides extract, and dissolving it in 200 μl of 75% ethanol solution to a final concentration of 7.5 mg / mL, the ethanol content of each of the above saponins was obtained. solution.

[0042] The control group used 200 μl 75% ethanol solution.

[0043] Methods: Human umbilical vein endothelial cells HUVEC and human ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a composition. The composition includes ginsenoside Rk1, ginsenoside Rg5, ginsenoside CK and a total ginsenoside ginseng root extract product; and the composition is an interleukin (IL)-6 conditioning agent. Applications of the composition in the preparation of drugs are also related.

Description

technical field [0001] The invention relates to a composition comprising the following components: ginsenoside Rk1, ginsenoside Rg5, ginsenoside CK, and total ginsenoside extract, wherein the composition is an interleukin (IL)-6 regulator. The invention also relates to the use of the composition in the preparation of medicaments. Background technique [0002] Interleukin-6 (IL-6) is a cytokine composed of 184 amino acids with a molecular weight of 21-26 kDa. Interleukin-6 is produced by various cells such as lymphocytes, monocytes, macrophages, vascular endothelial cells and tumor cells under the stimulation of various stimulating substances. Its biological properties include inducing B cells to produce antibodies, activating T cells, and accelerating the synthesis of other acute inflammatory proteins. On the other hand, the overexpression of interleukin-6 can lead to the occurrence of various inflammation-related diseases, including thrombocytosis, myeloma, Castleman's di...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/258A61P29/00A61P37/02A61P35/00A61P31/12A61P31/04A61P19/02A61P11/06A61K31/704
CPCA61K31/704A61K36/258A61P29/00A61P37/02A61P35/00A61P31/12A61P31/04A61P19/02A61P11/06A61K2300/00
Inventor 金英花李旭明王宇石
Owner 吉林海颐康达生物科技发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap