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Semen cassiae extract for reducing glucose and lipid and preparing and quality testing method thereof

A cassia seed and extract technology, applied in the field of medicine, can solve the problems of low active ingredient content, large dosage, lack of pharmacological activity support, etc., and achieve the effects of controllable quality, high extraction efficiency, and stable and reliable preparation method

Inactive Publication Date: 2019-08-09
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing cassia seed extraction and purification or content determination studies lack the support of pharmacological activity; or simple water-alcohol extraction is used for pharmacological activity research, so the dosage is large and the content of active ingredients is low

Method used

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  • Semen cassiae extract for reducing glucose and lipid and preparing and quality testing method thereof
  • Semen cassiae extract for reducing glucose and lipid and preparing and quality testing method thereof
  • Semen cassiae extract for reducing glucose and lipid and preparing and quality testing method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation process optimization process of Semen Cassiae extract of the present invention is as follows:

[0028] 1.1 Instruments and reagents

[0029] Agilent1260 high performance liquid chromatography, Huaxi HX-200 high-speed traditional Chinese medicine pulverizer (Zhejiang Yongkang Xi'an Hardware Medicine Factory), KH5200 ultrasonic cleaning machine (Kunshan Hechuang Ultrasonic Instrument Co., Ltd.), HH-S water bath (Zhengzhou Great Wall Science, Industry and Trade Co., Ltd.), JE602 Analytical Balance (Shanghai Puchun Measuring Instrument Co., Ltd.), and others are common laboratory instruments.

[0030] Cassia seeds were purchased from Tongrentang Pharmacy, Tianyi Hospital of Traditional Chinese Medicine, Jiefang Avenue, Wuhan, and identified as dried mature seeds of Cassia obtusifolia L. The specimens are stored in the Department of Pharmacognosy, Tongji College of Pharmacy, Huazhong University of Science and Technology. Orange cassiacin (batch number: 1606...

Embodiment 2

[0066] The preparation operation of Semen Cassiae extract of the present invention is as follows:

[0067] 2.1 Instruments and reagents

[0068] Same as 1.1.

[0069] 2.2 Experimental methods and results

[0070] Take three batches of cassia seeds (JMZ2017122010, JMZ2017122511, JMZ2017122512) each 1.8kg, crushed, soak in 14.4L of 80% ethanol 8 times the amount overnight, reflux extraction for 2 hours, extract once, filter, evaporate the solvent until there is no alcohol smell; extract The solution is made into a concentrated solution with a concentration of 0.6 g of the original drug per milliliter of the solution. Pack the D101 macroporous resin column with a column volume of 2L and absorb overnight. Wash 8L with water the next day, discard the eluent; elute 12L with 90% ethanol aqueous solution, collect the eluent, recover the solvent, evaporate to dryness in a water bath, dry in a vacuum, and grind finely to obtain the cassia seed extract, which is a brown-black powder. ...

Embodiment 3

[0075] The assay method of Semen Cassiae extract of the present invention is as follows:

[0076] 3.1 Instruments and reagents

[0077] The reference substance cassiaside (batch number: 20161216), cassiaside C (batch number: 20161215) was purchased from Chengdu Ansaisi Biotechnology Co., Ltd., sarubulin-6-O-β-D-gentiobioside (batch number: MUST -18030212) was purchased from Chengdu Mansite Biotechnology Co., Ltd., orange cassiacin-6-O-glucoside (batch number: 101712) was purchased from Jiangsu Yongjian Pharmaceutical Technology Co., Ltd., and other instruments and reagents were the same as 1.1.

[0078] 3.2 Experimental methods and results

[0079] 3.2.1 Preparation of the test solution

[0080] Take 20mg of cassia seed extract (JMZ2017122010) under item 2.2, weigh it accurately, put it in a 25ml volumetric flask, add methanol to ultrasonic to dissolve and dilute to the mark, shake well, and you get it.

[0081] 3.2.2 Preparation of reference solution

[0082] Respectively d...

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Abstract

The invention belongs to the field of medicines, and discloses a semen cassiae extract for reducing glucose and lipid and a preparing and quality testing method thereof. The extract is a product prepared through ethanol water extraction and large-pore resin purification with semen cassiae as the raw medicine material. The quality testing method of the extract includes the step of finitely detecting the property and content. In the step of finitely detecting the content, the contents of two anthraquinone components and three naphthopyranone components in the semen cassiae extract are measured at the same time through an efficient liquid chromatography method, and the methodological inspection result is good. It is provided through a pharmacological experiment that the semen cassiae extracthas a good glucose and lipid reducing function and can be used for preparing medicines and healthcare products for reducing glucose and lipid. The preparing method is stable and reliable, the qualitycan be controlled, the medicine effect is remarkable, and the semen cassiae extract has good development and application prospects.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to extracts of traditional Chinese medicinal materials, in particular to a preparation method and a quality detection method of cassia seed extracts and extracts with hypoglycemic and lipid-lowering effects, as well as their application in the preparation of pharmaceuticals and health care products. Background technique [0002] With the rapid development of social economy and changes in people's lifestyles, the intake of high-calorie and high-fat diets has increased, and the number of people suffering from the "three highs" of hyperglycemia, hyperlipidemia and hypertension is increasing. High blood sugar levels are caused by insulin production and dysfunction in the body, which can develop into diabetes and its complications; high blood lipid levels refer to metabolic abnormalities such as cholesterol, triglycerides, low-density lipoproteins, and high-density low-density lipoproteins in the bo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/482A61P3/10A61P3/06G01N30/88
CPCA61K36/482A61K2236/333A61K2236/39A61K2236/55A61P3/06A61P3/10G01N30/88G01N2030/8809
Inventor 陈家春王秋燕周杰文项志南童其林潘俊万落生
Owner HUAZHONG UNIV OF SCI & TECH
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