A traditional Chinese medicine extract composition and quality detection method for treating diabetes and diabetic nephropathy

A technology for diabetic nephropathy and extracts, which is used in drug combinations, pharmaceutical formulations, urinary system diseases, etc.

Active Publication Date: 2021-11-19
HUAZHONG UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The anti-diabetic drugs currently used clinically can delay the development of type 2 diabetes and its complications to a certain extent, but during use, side effects such as hypoglycemia, gastrointestinal reactions, and cardiovascular system diseases often occur

Method used

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  • A traditional Chinese medicine extract composition and quality detection method for treating diabetes and diabetic nephropathy
  • A traditional Chinese medicine extract composition and quality detection method for treating diabetes and diabetic nephropathy
  • A traditional Chinese medicine extract composition and quality detection method for treating diabetes and diabetic nephropathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0155] Embodiment 1: Apparatus and reagents: ultrafiltration system (molecular weight cut off is 1000Da), Millipore minipellicon. DZX-3 vacuum drying oven, Shanghai Fuma. LYO-0.5 freeze dryer, Shanghai Tofflon. The rest of the equipment are commonly used instruments in the laboratory. Papain (12U / mg), Guangxi Jiewoli. The rest of the reagents were purchased from Sinopharm Group and were of analytical grade (AR).

[0156] Coptis chinensis, Coptis chinensis Franch, a plant of the family Ranunculaceae, was purchased from the Coptis chinensis Production Cooperative in Jianzhuxi, Lichuan City, Hubei Province. Hubei Ophiopogon japonicus is the dry root tuber of Liriope spicata (Thunb.) Lour.var.prolifera Y.T.Ma, a plant of Liliaceae, purchased from the Hubei Ophiopogon japonicus medicinal material GAP planting base in Oumiao Town, Xiangfan City, Hubei Province. Bitter melon is the fresh and near-ripe fruit of Momordica charantia L., a plant of Cucurbitaceae, purchased from Diany...

Embodiment 2

[0170]1) Take 96 parts of Coptidis Rhizoma extract, 244 parts of Hubei Ophiopogon japonicus polysaccharide extract, 139 parts of Bitter Melon extract, and 49 parts of Semen Cassiae extract, grind and mix thoroughly to prepare Composition 1. The composition is a brownish-yellow powder with a very bitter taste. Among them, the proportion of Coptidis Rhizoma extract in the composition is 18.2%, that of Hubei Ophiopogon japonicus polysaccharide extract is 46.2%, that of bitter gourd extract is 26.3%, and that of Semen Cassiae is 9.3%. Each gram of composition 1 is equivalent to containing 1.04 grams of Coptidis Rhizome, 1.48 grams of Hubei Ophiopogon japonicus, 14.8 grams of fresh bitter gourd and 2.49 grams of Semen Cassiae.

[0171] 2) Take 98 parts of Coptidis Rhizome extract, 233 parts of Hubei Ophiopogon japonicus polysaccharide extract, 147 parts of Bitter Gourd extract, and 56 parts of Semen Cassiae extract, grind and mix well to prepare Composition II. The composition is ...

Embodiment 3

[0173] Example 3: The Chinese medicine extract composition of the present invention can effectively treat diabetes, and the screening and optimization process of the compatibility ratio of each extract in the composition is as follows:

[0174] 1. Instruments and reagents: see Examples 1 and 2 for the preparation and instruments of Chinese medicine extracts and their compositions. Coptidis extract batch number: HL2017032701, Hubei Ophiopogon japonicus polysaccharide extract batch number: HBMD2016011801, bitter gourd extract batch number: KG2017111201, cassia seed extract batch number: JMZ2017122010. Streptozotocin (STZ) was purchased from Sigma Aldrich (USA). Metformin hydrochloride tablets, Sino-US Shanghai Bristol-Myers Squibb. Total cholesterol (TC), total triglyceride (TG) kits were built in Nanjing.

[0175] The single herb extracts used in this experiment were tested for quality and all met the requirements. The specific detection method and detection results are show...

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Abstract

The invention belongs to the field of traditional Chinese medicine, and discloses a traditional Chinese medicine extract composition for treating diabetes and diabetic nephropathy, a preparation method and a quality detection method. The Hubei Ophiopogon japonicus polysaccharide extract obtained by decoction, impurity removal and ultrafiltration, the bitter gourd extract obtained by squeezing the bitter gourd juice obtained by combining it with ethanol cold soaking liquid, and the bitter gourd extract obtained after ethanol extraction of cassia seed and purified by macroporous resin Cassia seed extract: Provides a complete quality testing method for the composition and raw medicinal material extracts. Composition testing methods include property testing, moisture testing, extract testing, thin-layer chromatography testing, characteristic map testing and content limit testing, raw medicinal materials The detection methods of the extracts include the quality detection methods of the extracts of Coptis Rhizoma, Hubei Ophiopogon japonicus polysaccharides, bitter gourd and cassia seeds. The composition has good hypoglycemic and lipid-lowering effects, and has therapeutic effects on diabetes and diabetic nephropathy.

Description

[0001] This application requires submission of a Chinese patent application to the Patent Office of the State Intellectual Property Office of China on March 13, 2019, with the application number 201910187591.0, and the title of the invention "A traditional Chinese medicine extract composition and preparation method for treating diabetes and diabetic nephropathy". priority, the entire contents of which are incorporated by reference into this application. technical field [0002] The invention belongs to the field of traditional Chinese medicine and relates to a traditional Chinese medicine composition for treating diabetes, in particular to a traditional Chinese medicine extract composition for treating diabetes and diabetic nephropathy, a preparation method and a quality detection method thereof. Background technique [0003] Diabetes is a chronic disease that occurs due to the lack of or ineffective use of insulin in the body, resulting in elevated glucose levels in the bloo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/718A61P3/10A61P13/12A61K31/715
CPCA61K31/715A61K36/42A61K36/482A61K36/718A61K2236/19A61K2236/331A61K2236/333A61K2236/53A61K2236/55A61P3/10A61P13/12A61K2300/00
Inventor 陈家春周杰文项志南王秋燕童其林潘俊万落生
Owner HUAZHONG UNIV OF SCI & TECH
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