The invention relates to a production method for bamboo fiber, which comprises steps of cutting, softening, fiber splitting, scouring, bleaching, cleaning, dewatering, drying, enzyme treatment and the like. An optimal fiber splitting point after bamboo is softened can be fully developed by a fiber splitting separator, machining parameters can be adjusted according to requirement, and different slenderness rates of the fiber are obtained to make the processed bamboo fiber finer and more uniform than original fiber. The production process is reliable, the resource utilization rate is high, the processing cost is low, environmental pollution is low, and the product quality is stable. The fine bamboo pure fiber produced by the invention has the characteristics of uniform length and fineness, high intensity, good flexibility, high moisture absorbing and releasing performance, high air permeability and particularly remarkable bacteria resistance and deodorization, is a new generation natural functional fiber which is cheap and has high quality and superior cost performance, and can be widely applied to fields of textile, building, automobiles and the like.