Preparation method and application of dual-function biosensor HRP@ZIF-8/DNA
A biosensor, dual-function technology, used in biological testing, analysis by chemical reaction of materials, instruments, etc., to achieve good chemistry, good tolerance, and high selectivity.
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[0043] (1) Preparation of biomineralized HRP@ZIF-8
[0044] Accurately weigh 2 mg of horseradish peroxidase, add it to an aqueous solution of hexahydrate and zinc nitrate (50 mM, 500 μl), shake to dissolve the enzyme in the solution; imidazole solution, the two solutions were mixed thoroughly. The mixture was incubated in a constant temperature water bath at 30°C for 12 hours. After the reaction, the product was centrifuged and washed repeatedly. The resulting precipitate was HRP@ZIF-8. The principle is as figure 1 shown.
[0045] (2) Preparation of HRP@ZIF-8 / DNA hybrid system:
[0046]At room temperature, the HRP@ZIF-8 prepared in step (1) was dispersed in the Hepes buffer solution, and the concentration of HRP@ZIF-8 was 0.07 mg / mL; then, it was mixed with the fluorescently labeled aptamer solution, and the aptamer The concentration of the solution is 100nM; the volume ratio is 1:1; incubate at room temperature for 7min; until the fluorescence quenching is saturated to fo...
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