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Electrochemical sensors and methods of use thereof

A sensor and sensing technology, applied in the direction of biochemical equipment and methods, scientific instruments, instruments, etc., can solve problems such as prone to error and complicated calibration

Pending Publication Date: 2019-08-16
DIGITAL SENSING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Calibration of assays for the detection of target analytes in samples can be complex and prone to error due to a variety of factors such as sample handling, dilution errors, and / or interference from sample matrices such as milk, blood, or seawater

Method used

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  • Electrochemical sensors and methods of use thereof
  • Electrochemical sensors and methods of use thereof
  • Electrochemical sensors and methods of use thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1-S

[0662] Example 1 - Absorption of progesterone antibodies by SU8

[0663] Target

[0664] The binding of the binding agent progesterone antibody to the binding layer SU8 is demonstrated in an optical analysis format using horseradish peroxidase-P4 and 3,3',5,5'-tetramethylbenzidine.

[0665] method

[0666] The bonding layer SU8 was spin-coated (4000 rpm) onto the base material (glass slide) to obtain a layer approximately 0.2-0.4 μm thick. The surface was washed with ethanol and dried with nitrogen. The test area is defined using a 6mm diameter PMMA hole. P4 antibody (75 μl, 20 μg / ml in PBS) was added to the wells and incubated at 25°C for 1 hour. Excess antibody was removed by rinsing the surface with wash buffer (Tween PBS) (3 x 5 min). The surface was incubated for 1 hour with ethanolamine solution as a blocking agent (100 μl from a 1 M solution in Tris buffer, pH 8.5) to prevent non-specific binding. Assays were performed using 75 μl of horseradish peroxidase-P4 (200...

example 3

[0682] Example 3 - Detection of progesterone with an electrochemical platform where the sensor surface is a series of tips protruding from the binding area.

[0683] Target

[0684] Based on the optimized conditions in Examples 1 and 2, the P4 assay was performed using electrochemical measurements with the sensing surface (gold) within the binding area on the SU8 binding layer.

[0685] method

example 1

[0686] The method used in Example 1 was applied to an array of 20 μm tips protruding from the SU8 binding layer (as in Figure 12 shown). The antibody is applied in the form of a 3 μl drop only on the measuring electrode to create a binding area to which the binding agent is attached. The entire sensor surface was washed 6 times with wash buffer for 5 min before performing electrochemical measurements. In 3,3',5,5'-tetramethylbenzidine solution (0.1mg / ml, 0.006%H 2 o 2 ) for 3 minutes current measurement.

[0687] result

[0688] Figure 17 The P4 standard curve obtained by electrochemical assay with the sensing surface within the binding region (SU8) is shown.

[0689] in conclusion

[0690] The results demonstrate that electrochemical P4 determination can be performed with the sensing surface located within the binding region.

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Abstract

The invention is directed to the provision of a sensor comprising: a. a support substrate; b. at least one surface structure protruding from an upper surface of the support substrate, wherein the surface structure includes an electrode layer; c. a sensing surface on the electrode layer, wherein the sensing surface is adapted to contact a sample containing a target analyte; d. a binding region on the support substrate, wherein the binding region is separated from the sensing surface; wherein, in use, a binding agent attached to a binding layer at the binding region is also adapted to contact the sample containing the target analyte. The invention is also directed to methods of production and use of such sensors.

Description

technical field [0001] The present invention relates to sensors for detecting target analytes in solution. More specifically, the present invention relates to electrochemical sensors that detect the binding of target analytes to capture agents on arrays. Background technique [0002] Many medical, biological and biotechnological applications will benefit from more precise and reproducible measurements of biological or biochemical processes. However, converting information about biological events into detectable electronic signals is difficult due to the complexity of directly connecting electronic devices to biochemical environments. Due to their ability to directly convert biochemical events into electrical signals, electrochemical sensors offer an attractive means to achieve quantitative analysis of sample content. [0003] Electrochemical sensors are designed to be highly targeted to detect the presence (preferably concentration) of a target analyte (TA) in a sample. E...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N33/53
CPCC12Q1/005G01N33/54333G01N33/5438G01N27/3277C12Q2565/607
Inventor A·侯赛尼A·帕特里奇
Owner DIGITAL SENSING
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