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Method for improving viability and germination rate of pollen of catalpa bungei C.A.Mey.

A technology of pollen germination and viability, applied in biochemical equipment and methods, microbe measurement/inspection, plant cells, etc., can solve the problems of catalpa species in flowering period

Pending Publication Date: 2019-08-23
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] In view of the problems existing in the prior art, the object of the present invention is to provide a method for improving plant pollen viability and germination rate. The method provides pollen with high viability for catalpa hybrid pollination through the selection of pollen preservation conditions, and solves the problem of catalpa. The flowering period of the genus tree species does not meet the problem

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  • Method for improving viability and germination rate of pollen of catalpa bungei C.A.Mey.
  • Method for improving viability and germination rate of pollen of catalpa bungei C.A.Mey.
  • Method for improving viability and germination rate of pollen of catalpa bungei C.A.Mey.

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Embodiment 1

[0034] Materials and Methods

[0035] Test material: the test material is adult robust catalpa ( Catalpa bungei C. A. Meyer) and Catalpa ( C. fargesii Bur. f. duclouxii (Dode) Gilmour). Among them, catalpa trees are located in Yuntaishan National Forest Park in Lianyungang (referred to as LianCalpa) and in Nanjing Laoshan Forest Farm (referred to as LaoCalpa), neither of which can bear fruit by itself. Catalpa is located in the campus of Nanjing Forestry University (referred to as Catalpa), and it can bear fruit by itself.

[0036] From mid-April to early May 2015, collect large flowers or flower buds that are about to open at the full bloom stage, take out the anthers with tweezers, place them in a petri dish lined with sulfuric acid paper, and place them at room temperature (24°C±2°C) for about 2d, after the anthers crack and roll up naturally, the pollen is released and the pollen is collected. The pollen was put into a centrifuge tube and sealed, and after labeling...

Embodiment 2

[0058] Materials and Methods

[0059] Experimental materials: collection of materials: collection location: on the campus of Nanjing Forestry University; collection species: Dian catalpa, catalpa, catalpa, ash catalpa, golden tree; collection time: April-May 2007.

[0060]Material processing and storage: Immediately after the sample is collected, the pollen is processed in the laboratory, and the pollen is divided into two parts. One part is taken off the anther and placed in a petri dish covered with sulfuric acid paper to dry naturally for 2 days. After the pollen is scattered collect. The other part takes the pollen directly from the anther as a fresh sample. Afterwards, put the processed samples (including fresh and shade-dried samples) into centrifuge tubes, label them and store them in room temperature, 0°C, -20°C, -70°C, and -198°C (liquid nitrogen).

[0061] In addition, some morphological descriptions of fresh flowers were carried out, including flower size, flower ...

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Abstract

The invention discloses a method for improving the viability and germination rate of pollen of catalpa bungei C.A.Mey.. The method comprises the following steps of 1) collection of the pollen; 2) preservation of the pollen; 3) thawing; 4) in-vitro germination; 5) measurement of pollen storage capacity. By means of the method, the germination characteristics and storage characteristics of pollen ofcatalpa bungei C.A.Mey. and other catalpa tree species are systematically researched, reasons affecting the viability of the pollen are grasped, and the environmental conditions suitable for the storage of the in-vitro pollen of the catalpa bungei C.A.Mey. are determined; meanwhile, the temperature, sucrose concentration, polyethylene glycol concentration, pH value and other environmental factorspossibly related to the viability of the in-vitro pollen are comprehensively analyzed, and conditions most suitable for culturing the pollen of the catalpa bungei C.A.Mey. are obtained.

Description

technical field [0001] The invention relates to a method for improving the viability and germination rate of plant pollen, in particular to a method for improving the viability and germination rate of catalpa pollen. Background technique [0002] Catalpa ( Catalpa bungei C.A.Mey.) belongs to Bignoniaceae Catalpa genus tall deciduous tree species. It is a precious and high-quality timber tree species unique to my country and a famous garden ornamental tree species. It has a cultivation history of more than 3,000 years. It combines the advantages of timber, ornamental, and environmental protection. Loved by the masses, it has been known as the "King of Wood" since ancient times. Its material is excellent, and it is a high-quality and good material used in construction, furniture, shipbuilding, sculpture, musical instruments, crafts, military industry, etc. Catalpa is a beautiful tree, tall and straight, with luxuriant branches and leaves, colorful flowers and pleasant fragran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12Q1/02
CPCC12N5/0025C12N5/04G01N33/4833
Inventor 彭方仁谭鹏鹏王改萍梁有旺
Owner NANJING FORESTRY UNIV
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