Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Probe and detection method for HPV (Human Papillomavirus) detection

A probe and reaction technology, applied in the field of genetic engineering, can solve the problems of low efficiency and high cost

Inactive Publication Date: 2019-08-27
杭州链康医学检验实验室有限公司
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method has good specificity and high sensitivity, the efficiency is relatively low and the cost is also high.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Probe and detection method for HPV (Human Papillomavirus) detection
  • Probe and detection method for HPV (Human Papillomavirus) detection
  • Probe and detection method for HPV (Human Papillomavirus) detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0062] General implementation scheme: a kind of probe and detection method for HPV detection comprise the following steps:

[0063] (1) Prepare the probe;

[0064] (2) Prepare the DNA of cervical exfoliated cell samples with complete electrophoresis main band and no obvious degradation;

[0065] (3) Fragmentation of genomic DNA using ultrasonic physical fragmentation method;

[0066] (4) Purifying the fragmented product in step (3) with LC magnetic beads;

[0067] (5) constructing the fragmented DNA into an Illumina genome library;

[0068] (6) Amplify the genomic library by PCR reaction and use LC magnetic beads to purify the amplified product;

[0069] (7) Hybridizing the probe prepared in step (1) with the genomic library in step (6);

[0070] (8) capture the hybridization product with streptavidin magnetic beads and wash the non-specific binding;

[0071] (9) PCR amplification of target DNA fragments bound to the streptavidin magnetic beads;

[0072] (10) High-throug...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a probe and a detection method for HPV (Human Papillomavirus) detection. The preparation process of the probe requires two rounds of PCR reactions. The NGS technology-based detection method comprises the steps of preparing a DNA template, performing fragmentation on genomic DNA, purifying a fragmented product, constructing a genomic library, performing PCR amplification onthe genomic library, hybridizing the probe with the genomic library, capturing a hybridization product by magnetic beads, and performing high-throughput sequencing on the captured product and analyzing whether a sample to be tested is infected with HPV, the infection degree of the HPV, the integration location and the pathogenic mechanism of the HPV and the like. The probe and the method disclosedby the invention have the advantages of low cost, fast data analysis, high coverage depth, high detection accuracy and the like, and are suitable for early screening and postoperative diagnosis of diseases caused by the HPV, and provide guidance for personalized medication.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, and more specifically relates to a probe and a detection method for HPV detection. Background technique [0002] Human papillomavirus (Human Papillomavirus, HPV) is an epitheliophilic virus with high specificity. It is a small DNA virus with double-stranded closed circle and contains about 8000 base pairs. HPV can be integrated into the host cell genome to cause instability of the host cell genome, resulting in chromosomal inversion, translocation, deletion, and rearrangement, which makes the genome structure of the host cell abnormal, and can cause insertional mutagenesis leading to key tumor suppressor genes of the host cell. Abnormal inactivation or abnormal activation of oncogenes, and virus integration can also cause copy number variation in certain regions of the host cell genome, indirectly affecting the expression levels of some key oncogenes or tumor suppressor genes in host ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/70C12Q1/6806C12N15/11C12Q1/6869C12R1/93
CPCC12Q1/708C12Q1/6806C12Q1/6869C12Q2531/113C12Q2563/143C12Q2563/149C12Q2535/122
Inventor 郎秋蕾钱刚方超蔡霖霖殷楠楠刘歆李璐璐
Owner 杭州链康医学检验实验室有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com