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A method for establishing a rat model of autism by using fecal bacteria transplantation

A fecal bacteria transplantation and rat model technology, applied in animal husbandry and other directions, can solve the problems of complexity, unclear pathogenesis, and high scientific research cost, and achieve the effects of typical model symptoms, universal trial, and strong repeatability.

Active Publication Date: 2022-03-22
HENAN UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex etiology of ASD and the unclear pathogenesis, there is also a lack of strict animal models for experimental research.
From the analysis of the research status at home and abroad, in recent years, there are many ways to establish animal models of ASD and new progress has been made, but there are still some defects, such as time-consuming model preparation, overly complicated operations, poor reproducibility, and high research costs. And scientific research equipment has strict requirements, and cannot be widely promoted, etc.

Method used

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  • A method for establishing a rat model of autism by using fecal bacteria transplantation
  • A method for establishing a rat model of autism by using fecal bacteria transplantation
  • A method for establishing a rat model of autism by using fecal bacteria transplantation

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Embodiment 1

[0047] Embodiment 1 of the present invention discloses a method for establishing a rat model of autism by using fecal bacteria transplantation, which specifically includes the following steps:

[0048](1) Preparation of feces liquid:

[0049] After weighing, put the fresh feces of autistic patient donors in a sterile mixer, add sterile saline according to the sterile saline: feces = 5mL / g and stir; pass the feces through the apertures of 2.0mm and 1.0mm in turn , 0.5mm and 0.25mm four kinds of sterile stainless steel filter screens to filter the fecal juice;

[0050] B. Centrifuge the filtered fecal juice at 3000r / min for 15min, discard the supernatant, add normal saline to the original volume, mix well and then centrifuge, repeat this step 3 times to obtain the residue;

[0051] C. After weighing the residue, suspend it in normal saline to obtain 5g / mL fecal bacteria liquid.

[0052] The total treatment time is ≤60min. The fresh fecal bacteria liquid is directly transplante...

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Abstract

The invention discloses a method for establishing a rat model of autism by using feces and bacteria transplantation, which is characterized in that it specifically comprises the following steps: preparing feces and bacteria liquid: making feces from clinically diagnosed children with autism Fecal bacteria liquid; model establishment: The fecal bacteria liquid was injected into pregnant mice by enema, and the pregnant mice were then given birth to obtain offspring as autism model mice. The method disclosed by the invention has strong repeatability, high success rate, low mortality rate, more typical model symptoms, longer duration and low cost, and has practical value and significance.

Description

technical field [0001] The invention relates to the technical field of establishing an animal autism model, and more specifically relates to a method for establishing an autism rat model by using feces and bacteria transplantation. Background technique [0002] At present, autism spectrum disorder (ASD) is a relatively serious neurodevelopmental disorder that mostly occurs in infants and young children. Its typical features are communication impairment and imagination impairment, social dysfunction, and stereotyped repetitive interests and behaviors. , accompanied by motor coordination disorders. According to the latest data from the Centers for Disease Control and Prevention in the United States, the incidence of ASD has increased from 1 / 68 in 2014 to 1 / 59 by 2018. It has been reported in the literature that there are about 4 to 10 million autistic patients in my country, of which about 1 to 3 million are children. At this stage of research, it appears that autism spectru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 杨丽萍亓照耀袁海燕刘娟翟林林曹赟吕孟可王洪刚王启阳
Owner HENAN UNIV OF CHINESE MEDICINE
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