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Serial molecular markers and application thereof to pre-harvest sprouting gene molecular markers

A technology of molecular markers and ear germination, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of no breakthrough progress and large losses in wheat ear germination resistance breeding

Active Publication Date: 2019-08-30
HENAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because of the large losses caused, breeders from all over the world attach great importance to wheat ear germination, and have conducted a lot of research on its identification methods, resistance mechanisms, genetic mechanisms, and molecular markers. However, there has been no breakthrough in wheat ear germination resistance breeding.

Method used

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  • Serial molecular markers and application thereof to pre-harvest sprouting gene molecular markers
  • Serial molecular markers and application thereof to pre-harvest sprouting gene molecular markers
  • Serial molecular markers and application thereof to pre-harvest sprouting gene molecular markers

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Embodiment

[0044]It should be noted that the main technical idea of ​​this application is: through the phenotypic determination of whether the relevant gene fragments in the Jiejiemai T093 are introduced into the wheat grains to reduce the germination effect of the wheat grains, and further combined with SSR markers, QTL analysis and other technologies The specific design provides a series of molecular markers, which can lay the foundation for further breeding of wheat varieties resistant to panicle germination. Therefore, this embodiment briefly introduces the relevant experimental conditions as follows.

[0045] (1) Phenotypic identification of panicle germination resistance

[0046] Each wheat plant was tagged and marked at the flowering stage in April, and entered the wax ripening stage about 35 days after flowering. After the wheat ears were all chlorotic, 5 mature ears of wheat ears with the same main stem were taken from each material and dried outdoors for 7 days. The wheat ears...

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Abstract

The application of the invention belongs to the technical field of wheat molecular breeding, and particularly relates to serial molecular markers and an application thereof to pre-harvest sprouting gene molecular markers. The number of the molecular markers is 4, the molecular markers comprise an SSR marker and three KASP markers, wherein the SSR maker is an Xgwm383b marker, and the three KASP markers are A009711, A009180 and A009716. During variety breeding, the way of transferring excellent genes in aegilops tauschii to wheat is mainly to create a series of wheat / aegilops tauschii substitution line, and through distant hybridization means, aegilops tauschii chromosomes are introduced into wheat background. In accordance with the breeding method, practical F2 groups are used as an example, the serial molecular markers are designed to be used for detecting and tracking the situation of the aegilops tauschii chromosomes introduced into the wheat background, and further guiding pre-harvest sprouting resistant new species cultivation, and the serial molecular markers have better practical value.

Description

technical field [0001] The application belongs to the technical field of wheat molecular breeding, and specifically relates to a series of molecular markers and their application in wheat ear germination gene molecular markers. Background technique [0002] Pre-harvest sprouting (PHS) is a phenomenon of germination on ears caused by rainy or humid environment after physiological maturity of wheat before harvest. The phenomenon of ear germination not only affects the yield of wheat, but also seriously affects the quality of processing, seed storage and next year's sowing quality, and then causes economic losses in varying degrees. [0003] Wheat ear germination is a worldwide climate disaster. Ear germination occurs in wheat producing areas all over the world, causing serious losses. Ear germination in my country mainly occurs in the Yangtze River Basin, Southwest wheat region, and Northeast spring wheat region. Large-scale ear germination has also occurred many times in Gua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 贺洁李锁平张大乐苏亚蕊李玉阁高安礼
Owner HENAN UNIVERSITY
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