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Method for detecting whether pig farm flies and their living environment carry African swine fever virus

An African swine fever virus and living environment technology, which is applied in the field of detecting whether flies and their living environment in pig farms carry African swine fever virus, which can solve the problems of undisclosed or prompted, inability to detect and detect, and reduce the possibility of recurrence. Effect

Active Publication Date: 2020-12-04
湖南省动物疫病预防控制中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, after pig farms are infected with African swine fever virus, mosquitoes and flies will spread and spread the virus, so that African swine fever virus remains in the environment where mosquitoes and flies live. , disinfection to eliminate the virus carried by mosquitoes as much as possible, but for mosquitoes, especially whether the African swine fever virus in the living environment of mosquitoes still exists or is completely killed, it cannot be detected and detected by means of existing technologies. Or the prior art does not disclose or suggest how to obtain African swine fever virus detection samples in the living environment of mosquitoes and flies, so as to obtain detection results accurately and quickly

Method used

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  • Method for detecting whether pig farm flies and their living environment carry African swine fever virus
  • Method for detecting whether pig farm flies and their living environment carry African swine fever virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Select a diseased pig farm announced by the Ministry of Agriculture and Rural Affairs as the place to be tested. After 2 months of unblocking, the method of the present invention is used to detect whether the pig farm flies and their living environment carry African swine fever virus. The specific steps are as follows:

[0029] Step 1. Kill flies

[0030] Use fenitrothion to kill flies in the living area, garbage disposal area and waste disposal area of ​​the pig farm, and collect all the dead flies in each area of ​​the pig farm. A total of about 89 dead flies were collected;

[0031] Step 2. Detection

[0032] The fly corpses collected in step 1 were mixed and crushed, and then samples were taken to quantitatively detect the African swine fever pathogen by fluorescent PCR. The test results are shown in Table 1;

[0033] Table 1 Detection results of African swine fever virus in fly cadavers

[0034] group result dead fly 36.48 negative control ...

Embodiment 2

[0041] Select a diseased pig farm announced by the Ministry of Agriculture and Rural Affairs as the detection site. After the blockage is lifted for a period of time, the method of the present invention is used to detect whether the flies in the pig farm and their living environment carry African swine fever virus to confirm the disinfecting effect. The specific steps are as follows:

[0042] Step 1. Kill the flies

[0043] In the living area, garbage disposal area, and waste disposal area of ​​the pig farm, fenitrothion was used to kill the flies in the whole field, and all the dead flies in each area of ​​the pig farm were collected, and a total of about 54 dead flies were collected;

[0044] Step 2. Detection

[0045] The fly corpses collected in step 1 were mixed and crushed, and then samples were taken to detect the African swine fever pathogen by fluorescent PCR. The test results are shown in Table 3;

[0046] Table 3 Detection results of African swine fever virus in fl...

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Abstract

The invention discloses a method for detecting whether swine flies and living environment carrying African swine fever virus. The method comprises the following steps: step 1: killing the fly: killingthe flies of a pig living area, a garbage processing area and a waste processing area, and collecting all the fly bodies in various areas of a pig farm; step 2: detecting: mixing and triturating thefly bodies collected in step 1, and detecting the pathogens of African swine fever in the fly bodies, if the detection result is negative, the flies and flies in the living environment of the pig farms do not carry the African swine fever virus, and then entering a step 3 to eliminate the existence of false negative detection results; if the detection result is positive, resulting that the flies and fly living environment in the pig farm carry African swine fever virus; and step 3: putting the fruit fly into the test. The method can accurately and quickly detect whether the flies and flies inliving environment of the pig farm carry the African swine fever virus, so as to provide a basis for whether the pig farm is suitable for resumption of production.

Description

technical field [0001] The invention belongs to the technical field of prevention and detection of African swine fever, and in particular relates to a method for detecting whether flies in pig farms and their living environment carry African swine fever virus. Background technique [0002] African swine fever (African swine fever, ASF) is an acute, febrile, highly contagious infectious disease of pigs caused by African swine fever virus infection. It is characterized by organ bleeding and high mortality. African swine fever originated in Africa and has been prevalent in East Africa for a long time in the past. According to the analysis of literature records, the African swine fever epidemic can be traced back to the early 20th century. In 1921, the African swine fever epidemic was first confirmed in the Kenyan country in East Africa. Since then, the disease has spread from East Africa, South Africa, Central Africa, West Africa, West Europe, and the Americas to Eastern Eur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/686C12Q1/24C12R1/93
CPCC12Q1/24C12Q1/686C12Q1/701C12Q2563/107
Inventor 林源何世成王昌建刘道新喻正军胡巧云周小双
Owner 湖南省动物疫病预防控制中心
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