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Typing method

A chromosome and nucleic acid technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as difficulties in the early stage

Pending Publication Date: 2019-09-10
牛津生物动力有限责任公司
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  • Description
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Problems solved by technology

[0002] Current diagnosis of neurodegenerative diseases, especially in the early stages, is often difficult

Method used

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specific Embodiment approach

[0145] The EpiSwitch TM The platform technology detects epigenetic regulatory signatures of regulatory changes between normal and abnormal conditions at loci. The EpiSwitch TM The platform identifies and monitors the basic epigenetic level of gene regulation associated with the regulatory high-level structure of human chromosomes, also known as chromosome conformation signatures. Chromosomal signatures are unique critical steps in the cascade of genetic dysregulation. They are advanced biomarkers with a unique set of advantages over biomarker platforms utilizing late epigenetic and gene expression biomarkers such as DNA methylation and RNA profiling.

[0146] EpiSwitch TM Array analysis

[0147] The custom EpiSwitch TM The array screening platform has 4 densities of 15K, 45K, 100K and 250K unique chromosome conformations, and each chimeric fragment is repeated 4 times on the array, so that the effective densities are 60K, 180K, 400K and 1 million, respectively.

[0148] ...

Embodiment

[0183] The inventors characterized the epigenetic profile of patients with neurodegenerative diseases based on observations of AD (Alzheimer's disease) positive patients and age-matched AD negative controls. Details of the genes for which AD markers have been identified are shown in Table 1 together with the associated probe sequences and positions (Table 2). Sample-specific EpiSwitch using 50 μL of blood per extraction reaction TM extraction process. Briefly, the samples were fixed with formaldehyde, incubated for 15 minutes and quenched with glycine. Samples were lysed and nuclei were purified using density cushion centrifugation. Proximal ligation was performed with restriction enzymes TaqI and T4 DNA ligase. For each extraction, the protein and RNA content was removed by proteinase K.

[0184] Nested PCR was performed using 13 μL of the sample dilution series as a template. The PCR products were analyzed using the LabChip GX Touch HT (Perkin Elmer) high-throughput gel...

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Abstract

The invention provides a method of determining the epigenetic chromosome interactions which are relevant to an epigenetic test for a neurodegenerative condition.

Description

technical field [0001] The present invention relates to detecting chromosomal interactions. Background technique [0002] Current diagnosis of neurodegenerative diseases, especially in the early stages, is often difficult. Usually the diagnosis occurs when symptoms such as poor mental ability begin to appear. Contents of the invention [0003] The present inventors have identified regions of the genome where chromosomal interactions are associated with neurodegenerative diseases. The inventors' work allowed the typing of individuals into subgroups with distinct neurodegenerative characteristics based on chromosomal interactions. [0004] Accordingly, the present method provides a method for determining said epigenetic chromosomal interactions in relation to an epigenetic test for a neurodegenerative disease, wherein said test distinguishes subgroups comprising making a first group from said subgroup The nucleic acid is contacted with a second set of nucleic acids repres...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6827C12Q1/6883C12Q2521/301C12Q2521/501C12Q2523/101C12Q2565/501C12Q2600/118
Inventor 阿鲁尔·塞尔瓦姆·拉马达斯埃万·亨特亚历山大·阿库利切夫
Owner 牛津生物动力有限责任公司
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