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Preparation of a composite hydrogel material and method for printing composite scaffolds
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A technology of composite hydrogel and composite scaffold, which is applied in the field of tissue engineering and regenerative medicine, can solve the problems affecting application and single material forming method, and achieve the effects of avoiding denaturation, simple and quick preparation, and low price
Active Publication Date: 2022-07-08
SHANGHAI UNIV
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However, its material forming method is single, which affects its application
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Embodiment 1
[0030] In this example, see figure 1 and figure 2 As shown, a preparation of a composite hydrogel material and a method for printing a composite scaffold. The composite hydrogel is prepared by using egg white and sodium alginate as materials, and the composite scaffold is prepared by extrusion 3D printing and chemical cross-linking; including the following step:
[0031] a. Preparation of composite hydrogel materials:
[0032] First, put the eggs into deionized water for surface cleaning, then put them into 75% medical alcohol, and irradiate them with UV light for 2 hours for disinfection, and then put them into PBS for washing three times; In the clean bench, break the eggs to separate the egg whites and yolks, put the obtained egg whites in a centrifuge tube, and store them in a refrigerator at 4°C for later use;
[0033] Next, take a set amount of sodium alginate and add it to the beaker, add deionized water, and fully stir it to completely dissolve in deionized water t...
Embodiment 2
[0044]This embodiment is basically the same as the first embodiment, and the special features are:
[0045] A preparation method of a composite hydrogel material and a composite scaffold printing method, comprising the following steps:
[0046] a. Preparation of composite hydrogel materials:
[0047] First, put the eggs into deionized water for surface cleaning, then put them into 75% medical alcohol, and irradiate them with UV light for 2 hours for disinfection, and then put them into PBS for washing three times; In the clean bench, break the eggs to separate the egg whites and yolks, put the obtained egg whites in a centrifuge tube, and store them in a refrigerator at 4°C for later use;
[0048] Next, take a set amount of sodium alginate and add it to the beaker, add deionized water, and fully stir it to completely dissolve in deionized water to prepare a sodium alginate solution with a concentration of 5±0.5%;
[0049] Next, weigh a set amount of gelatin powder and place ...
Embodiment 3
[0058] This embodiment is basically the same as the first embodiment, and the special features are:
[0059] A preparation method of a composite hydrogel material and a composite scaffold printing method, comprising the following steps:
[0060] a. This step is the same as the first embodiment;
[0061] b. Preparation of cross-linking agent:
[0062] Weigh a set amount of barium chloride powder and place it in a beaker, add deionized water, fully stir to dissolve, and obtain a barium chloride solution with a concentration of 5±0.1% as a crosslinking agent;
[0063] c. This step is the same as the first embodiment.
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Abstract
The invention discloses a preparation method of a composite hydrogel material and a composite scaffold printing method. A composite hydrogel prepared by mixing egg white and a forming material is used as a biological material, and the composite hydrogel is prepared by using a composite process method of 3D printing and cross-linking forming. out the composite bracket. The invention realizes the directional printing of the egg white solution by mixing the egg white and the forming material to prepare and print; and the method does not affect the properties of various proteins contained in the egg white, improves the biological properties of the scaffold, and helps to promote the scaffolding. Vascularization. At the same time, the egg white has good water retention, that is, the composite scaffold prepared by mixing the egg white and the forming material provides a good environment for cell growth. In addition, egg whites and shaping materials are common and readily available materials, and the methods of preparation are simple and efficient. Because, the egg white and forming material composite hydrogel material and composite scaffold of the present invention have very broad application prospects in the fields of tissue engineering and regenerative medicine.
Description
technical field [0001] The invention relates to a preparation method of a composite hydrogel material and a composite scaffold printing method, which are applied to the fields of tissue engineering and regenerative medicine. Background technique [0002] In recent years, tissue engineering has developed rapidly in the field of biomedicine, and 3D bioprinting technology has become the focus of research, but bioprinting ink has become a difficult point affecting the development of 3D bioprinting. As a common bioprinting material, hydrogels are widely used in tissue engineering. However, it is difficult for a single hydrogel material to meet the corresponding needs of biomedicine, such as biocompatibility and biomechanical properties. Therefore, the preparation of composite hydrogels and the printing of their scaffolds have become a research hotspot. [0003] Protein-based materials hold great promise in the field of biomaterials due to their intrinsic and unique properties, ...
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