Dual-mode tumor imaging nanoprobe suitable for MRI/NIR-II, and preparation method and application thereof

A nanoprobe and tumor imaging technology, applied in the field of biomedical materials, can solve the problems of complex toxicity of dual-modal imaging material synthesis, and achieve high industrial application prospects, simple synthesis route, and good imaging effect.

Active Publication Date: 2019-09-17
SUZHOU INSTITUE OF WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The synthesis of general dual-modal imaging materials is complex and has certain toxicity. The most concerned thing is the imaging effect and targeting effect of the material.

Method used

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  • Dual-mode tumor imaging nanoprobe suitable for MRI/NIR-II, and preparation method and application thereof
  • Dual-mode tumor imaging nanoprobe suitable for MRI/NIR-II, and preparation method and application thereof
  • Dual-mode tumor imaging nanoprobe suitable for MRI/NIR-II, and preparation method and application thereof

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Effect test

Embodiment 1

[0046] A dual-modal tumor imaging nanoprobe suitable for MRI / NIR-II, composed of apo-lactoferrin, Mn 2+ . 2+ And it is coupled with the amino group on PEG and the carboxyl group on CH1055, and the molecules are cross-linked through aldehyde groups and amino groups.

[0047] figure 1 It is a schematic structural diagram of the dual-mode tumor imaging nanoprobe of the present invention; wherein, for lactoferrin, for manganese ions, for the CH1055 molecule, for PEG, For iron ions; figure 2 It is a schematic structural diagram of the synthesis process of the dual-modal tumor imaging nanoprobe of the present invention.

[0048] The particle size of the dual-mode tumor imaging nanoprobe is 150-170nm.

Embodiment 2

[0050] A method for preparing a dual-mode tumor imaging nanoprobe suitable for MRI / NIR-II, comprising the following steps:

[0051] (1) The raw material lactoferrin is prepared into an aqueous solution of a certain concentration, and the iron ion on the lactoferrin is removed by dialysis at 15° C. with an acid solution with a pH of 2, and freeze-dried to obtain apo-lactoferrin Apo-Lf;

[0052] (2) Dissolve the Apo-Lf obtained in step (1) in a mixed solution of a certain amount of manganese salt and carbonate, stir at 20°C until the solution becomes a suspension, and then use a certain concentration of carbonate solution Dialyze with water to remove impurities, and freeze-dry to obtain iron-free plus manganese lactoferrin Lf-Mn 2+ ;

[0053] (3) In an anhydrous and oxygen-free environment, a certain amount of PEG is dissolved in DMF, and a certain amount of EDCI, NHS, and the Lf-Mn obtained in the step (2) are added successively. 2+ , DIPEA was stirred at room temperature unt...

Embodiment 3

[0063] A method for preparing a dual-mode tumor imaging nanoprobe suitable for MRI / NIR-II, comprising the following steps:

[0064] (1) The raw material lactoferrin is prepared into an aqueous solution of a certain concentration, and the iron ion on the lactoferrin is removed by dialysis at 25° C. with an acid solution having a pH of 6, and freeze-dried to obtain apo-lactoferrin Apo-Lf;

[0065] (2) Dissolve the Apo-Lf obtained in step (1) in a mixed solution of a certain amount of manganese salt and carbonate, stir at 30°C until the solution becomes a suspension, and then use a certain concentration of carbonate solution Dialyze with water to remove impurities, and freeze-dry to obtain iron-free plus manganese lactoferrin Lf-Mn 2+ ;

[0066] (3) In an anhydrous and oxygen-free environment, a certain amount of PEG is dissolved in DMF, and a certain amount of EDCI, NHS, and the Lf-Mn obtained in the step (2) are added successively. 2+ , DIPEA was stirred at room temperature u...

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Abstract

The invention relates to the technical field of biomedical materials, and specifically relates to a dual-mode tumor imaging nanoprobe suitable for MRI/NIR-II as well as a preparation method and application thereof. The dual-mode tumor imaging nanoprobe suitable for MRI/NIR-II is composed of deioned lactoferrin, Mn<2+>, PEG and CH1055, wherein the deioned lactoferrin is a vector which composes complexes with the Mn<2+> and couples with amino groups on the PEG as well as carboxyl groups on the CH1055; and the molecules are cross-linked by formyl groups and amino groups. The dual-mode tumor imaging nanoprobe suitable for MRI/NIR-II disclosed by the invention is a brand new compound feasible for dual-mode imaging, and has a fluorescence emission wavelength in the second region of near infrared; moreover, the dual-mode tumor imaging nanoprobe is non-toxic, good in biocompatibility, and feasible for easy absorption and metabolizsm by organisms. In addition, MR imaging is enabled by complexes with manganese ions so as to allow clear imaging effects. Being entrapped, the dual-mode tumor imaging nanoprobe can be used for detecting-imaging of various diseases, such as tumor and the like.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a dual-mode tumor imaging nanoprobe suitable for MRI / NIR-II, a preparation method and an application. Background technique [0002] Cancer (also known as malignant tumor) is a serious threat to human health. Due to the limitation of medical technology level, there is currently no effective treatment for advanced cancer, so early diagnosis of cancer is particularly important for patients. Early detection and timely treatment can significantly improve the survival rate of cancer patients. The emergence of molecular imaging technologies such as non-invasive living animal fluorescence imaging technology has opened up a new development path for the early diagnosis of cancer. [0003] MRI has the advantages of noninvasiveness, high spatial resolution, and good soft tissue contrast, and has been widely used clinically to provide qualitative diagnosis for early tumor growth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/12A61K49/14
CPCA61K49/0002A61K49/0021A61K49/0056A61K49/0054A61K49/14A61K49/126
Inventor 肖玉玲洪学传周晖杨涵
Owner SUZHOU INSTITUE OF WUHAN UNIV
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