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Codonopsis pilosula quality detection method based on spectrum-effect relationship

A quality inspection method, the technology of Codonopsis pilosula, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of scientific and perfect quality control, strong reproducibility, and strong objectivity

Active Publication Date: 2022-04-01
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is still a lack of effective and scientific quality assessment methods for the quality of Codonopsis pilosula, especially the method for detecting the quality of Codonopsis pilosula

Method used

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  • Codonopsis pilosula quality detection method based on spectrum-effect relationship
  • Codonopsis pilosula quality detection method based on spectrum-effect relationship
  • Codonopsis pilosula quality detection method based on spectrum-effect relationship

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Preparation of Codonopsis Codonopsis Water Elution Part

[0042]Take Codonopsis 40 mesh powder 100g, add 10 times the amount of water to mix, heat reflux extraction three times, combine the extracts and concentrate under reduced pressure. Add absolute ethanol to the concentrated solution until the ethanol concentration is 80%, alcohol precipitation overnight at 4°C, filter, dissolve the precipitate in water and repeat the previous step (precipitation is Codonopsis polysaccharide). The two filtrates were combined, concentrated under reduced pressure to obtain a fluid extract, which was dissolved in an appropriate amount of water, passed through a D101 macroporous resin column, and eluted with water to obtain the water fraction of Codonopsis pilosula.

Embodiment 2

[0043] Example 2. Establishment of Fingerprints of Codonopsis Codonopsis Water Elution Parts

[0044] 1) Solution preparation: Weigh the extract of Codonopsis pilosula eluted with water (containing 2.5g of crude drug), add pure water and dilute to 7.0mL, absorb 1.0mL and pass through a 0.45μm filter membrane to obtain the subsequent filtrate to obtain the test solution; D-glucose, D-fructose, D-galactose, D-xylose reference substance 5.0mg each, dilute to 1.0mL with pure water, filter to obtain each monosaccharide reference substance solution.

[0045] 2) Chromatographic conditions: TSK Oligo PW (7.8mm×30cm) chromatographic column is used. The mobile phase was 0.08mol / L ammonium formate isocratic elution for 15min; the flow rate was 0.8mL / min; the column temperature was 15°C; the injection volume was 1μL. The detector is an Alltech 3300 ELSD detector, the temperature is 60°C, and the gas flow rate is 2.0 L / min.

[0046] 3) Methodological investigation:

[0047] a. System ad...

Embodiment 3

[0076] Example 3. Pharmacodynamic study on the water-eluted part of Codonopsis pilosula:

[0077] 1) Solution preparation:

[0078] a. 100% rhubarb decoction: take an appropriate amount of rhubarb, soak in 10 times of water for 1 hour, heat and boil for 15 minutes, filter, add 8 times of water to the residue and boil for 15 minutes, filter, combine the filtrates, and condense into 100% rhubarb decoction ( Contain crude drug 1g / mL);

[0079] b. Codonopsis water-eluted part suspension: take 20 batches of Codonopsis pilosula water-eluted part extract in proportion to prepare 0.4g / mL Codonopsis radix water-eluted part suspension;

[0080] c. Carbon powder suspension: weigh 50g of gum arabic, add 350mL of water, boil until the solution is transparent, add 25g of activated carbon fine powder, boil for 3 times, let cool, add water to make up to 500mL.

[0081] 2) Model establishment and administration method: Mice were randomly and equally divided into blank control group, model gr...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine quality standard detection, and discloses a quality detection method of Codonopsis pilosula, including the preparation of water-eluted parts of Codonopsis radix, establishment of fingerprints of the water-eluted parts of Codonopsis radix, and spleen-invigorating pharmacodynamic research on the water-eluted parts of Codonopsis radix; The gray relational analysis method was used to establish the spectrum-effect relationship between the common peaks in the fingerprint of the water-eluted part of Codonopsis pilosula and the efficacy of invigorating the spleen; according to the spectrum-effect relationship, a quality standard was established to evaluate the quality of Codonopsis pilosula. The method for detecting the quality of Codonopsis radix Codonopsis in the present invention is based on the fingerprint of the water-eluted part of Codonopsis radix, taking the effect of invigorating the spleen of the eluted part of Codonopsis radix water as the main content, and using the gray correlation analysis method to analyze the spectrum-effect relationship of the eluted part of Codonopsis radix water, And thus establish a scientific and perfect quality standard of Codonopsis ginseng. The invention has the advantages of convenience and conciseness, strong objectivity, strong reproducibility, etc., makes the quality control of the Codonopsis pilosula more scientific and perfect, and establishes a method for evaluating the quality of the Codonopsis pilosula that is clearly related to the drug effect and specifically quantified.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine quality standard detection, and in particular relates to a quality detection method of Codonopsis pilosula, especially a quality detection method of Codonopsis pilosula and a method for establishing a detection model based on spectrum-effect relationship. Background technique [0002] Codonopsis pilosula is the root of Codonopsispilosula(Franch.)Nannf., C.PilosulaNannf.var.modesta(Nannf.)L.T.Shen or C.tangshenOliv., a plant of Campanulaceae. It is sweet and flat, and returns to the spleen and lung meridian . Codonopsis pilosula can invigorate the spleen and lungs, nourish blood and promote body fluid. It is mainly used in clinically for syndromes of spleen and lung qi deficiency, qi and fluid damage, and qi and blood deficiency. The merit of warming and tonifying has the power of Ganping and clearing the lungs." [0003] Codonopsis pilosula nourishes qi and nourishes blood, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 范罗嫡于玲刘美霞戚进余伯阳胡明华马方励
Owner INFINITUS (CHINA) CO LTD
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