Focusing method and device for microscope, terminal equipment and storage medium
A focusing method and microscope technology, applied in microscopes, installations, optics, etc., can solve the problems of reducing imaging quality and consuming a lot of time, and achieve the effect of ensuring quality and shortening focusing time.
Active Publication Date: 2019-09-17
SHENZHEN SMART IMAGING HEALTHCARE
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[0003] In view of this, the embodiment of the present invention provides a microscope focusing method, device, terminal equipment and storage medium to solve the problem in the prior art that the focusing of a fluorescence microscope takes a lot of time and reduces the imaging quality
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[0025] In the following description, specific details such as specific system structures and technologies are presented for the purpose of illustration rather than limitation, so as to thoroughly understand the embodiments of the present invention. It will be apparent, however, to one skilled in the art that the invention may be practiced in other embodiments without these specific details. In other instances, detailed descriptions of well-known systems, devices, circuits, and methods are omitted so as not to obscure the description of the present invention with unnecessary detail.
[0026] At present, the existing autofocus method needs to set a large moving range of the stage in order to ensure that the stage passes the focus point, but it takes a lot of time to focus the fluorescence microscope, and the fluorescent sample will be quenched with time. , ultimately degrading the image quality.
[0027] In this embodiment, the displacement range of the stage is adjusted, and t...
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The invention is applicable to the technical field of microscopy imaging, and provides a focusing method and device for a microscope, terminal equipment and a storage medium. The method comprises the steps of: controlling an object stage to move in a first displacement range smaller than a movement range of the object stage along a direction which faces or is away from an object lens, acquiring a group of first images of a sample on the object stage in the moving process of the object stage, and recording position information of the object stage when acquiring each first image; acquiring a definition value of each first image, and according to the definition value of each first image and the position information, generating a first focusing curve; judging whether the position information corresponding to a wave peak of the first focusing curve is within a preset offset of a maximum value or a minimum value of the first displacement range; and if no, controlling the object stage to move to a position represented by the position information corresponding to the wave peak. A problem that focusing of a fluorescence microscope needs to consume a large amount of time so as to reduce imaging quality is solved, shortening on focusing time is implemented, and quality of microscopy imaging is ensured.
Description
technical field [0001] The invention belongs to the technical field of microscopic imaging, and in particular relates to a microscopic focusing method, device, terminal equipment and storage medium. Background technique [0002] Fluorescence microscopy imaging has important applications in the field of biomedical research, and the autofocus method of fluorescence microscopy is the key to fluorescence microscopy imaging. In order to ensure the successful focusing of the fluorescence microscope, the existing autofocus method needs to ensure that the stage must pass through the focal point when the in-focus image is collected. In order to ensure that the stage passes through the focal point, it is necessary to set a larger movement range of the stage, but it takes a lot of time to focus the fluorescence microscope, and the fluorescent sample will be quenched with time, which will eventually reduce the imaging quality . Contents of the invention [0003] In view of this, the...
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Login to View More IPC IPC(8): G02B21/24G02B7/36
CPCG02B21/244G02B7/36
Inventor 刘旋权申文刘远明李宏军
Owner SHENZHEN SMART IMAGING HEALTHCARE