A method for improving the hatching rate of fertilized eggs of Macrobrachium japonicus in vitro

Abstract

The invention discloses a method for improving the hatching rate of fertilized eggs of Macrobrachium japonicus in vitro, which comprises the following steps: Step 1, selecting Macrobrachium prawns, the body length of the Macrobrachium prawns caught in the first step is larger than 4 cm, and the amount of eggs is larger than 1500 ; Step 2, the cultivation of Macrobrachium prawns, in the cultivation pond described in step 2, plastic aquatic plants are placed for Macrobrachium prawns to inhabit, and the culture water is changed every 2 days, and the amount of water changed is 1 / 3 of the total water volume; Step 3, fertilized eggs Separation, the fertilized eggs described in step 3 are the fertilized eggs when Macrobrachium ovale is cultivated until two black eyespots appear in the eggs; step 4, hatching; step 5, hatching management. The method for improving the in vitro hatching rate of Macrobrachium japonicus fertilized eggs can effectively reduce the in vitro hatching period of fertilized eggs, reduce the cumbersome operations in the hatching process, increase the convenience of management in the hatching process, and can effectively prevent the fertilized eggs from being hatched in vitro. Saprolegniasis was infected after the body and during the hatching process, thereby effectively improving the in vitro hatching rate of fertilized eggs of Macrobrachium prawn.

Description

technical field [0001] The invention relates to the technical field of artificial breeding of shrimps in aquaculture, in particular to a method for improving the in vitro hatching rate of fertilized eggs of Macrobrachium japonicus. Background technique [0002] Macrobrachium japonicus belongs to the decapod order, the long arm shrimp family, and the genus Macrobrachium, commonly known as freshwater shrimp or river shrimp, and is widely distributed in freshwater waters in my country; the shrimp has the advantages of tender meat, delicious taste, and rich nutrition, and is popular among consumers. It is an important economic shrimp in my country. During the artificial breeding process of Macrobrachium prawns, due to the long-term use of the "catch the big and keep the small" round-catching market model, the size of the parental bodies used for reproduction in the later period has been continuously reduced, which has virtually formed a growth disadvantage of Macrobrachium prawns...

AI Technical Summary

Problems solved by technology

However, this technology not only requires special treatment of hatching water, but also the hatching time is as long as 260-650 hours, the daily management work of the hatching process is relatively heavy, and long-term hatching is prone to operational errors, resulting in large fluctuations in the hatching rate; in this technology Under certain conditions, the hatching rate of fertilized eggs in vitro is 65.4% to 70.8%, with an average of 67.9%, which is still at a low level; The Key Technology of Macrobrachium Fertilized Egg Hatching in Vitro

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