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Functional wheat biotechnology breeding method for improving selenium content in wheat

A kind of biotechnology and wheat technology, applied in the direction of botany equipment and methods, other methods of inserting foreign genetic materials, recombinant DNA technology, etc., can solve the problems of low selenium content in wheat and non-genus

Inactive Publication Date: 2019-09-20
重庆汇鑫云强实业集团有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the selenium content of wheat is not high, and it does not belong to the category of selenium-enriched agricultural products stipulated by the state

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 of the present invention: a functional wheat biotechnology breeding method for increasing the selenium content of wheat, comprising the following steps: S1: selecting selenium-containing edible crops as a donor; S2: selecting wheat as a parent; S3: extracting the DNA of selenium-containing edible crops; S4: introducing the DNA of selenium-containing edible crops into the wheat parent; S5: collecting the distant hybrid seeds of the wheat parent in step S4; S6: selecting and breeding the distant hybrid seeds collected in step S5 Seeds of new strains with stable growth were obtained after generations.

[0033] The method for extracting DNA comprises the following steps:

[0034] S31: Weigh 0.2 g of the first to fourth young leaves of the donor seedling, remove the DNA contamination on the surface, put them in a mortar and grind them together with liquid nitrogen to form a fine powder;

[0035] S32: Transfer the fine powder of the donor into a 2ml centrifuge t...

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PUM

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Abstract

The invention discloses a functional wheat biotechnology breeding method for improving selenium content in wheat. The method includes the following steps: S1. selecting edible crops containing selenium as donors; S2. selecting wheat as a parent; S3, extracting the DNA of the selenium-containing edible crops; S4, introducing the DNA of selenium-containing edible crops into a wheat parent; and S5. collecting distant hybrid seeds of the wheat parent in the step S4. The seeds bred by the method have selenium content as high as 0.1 mg / kg in rice. By adopting a distant hybridization technology, the characteristics of selenium-containing crops are transferred to wheat to improve the selenium content in wheat. The method selects Sichuan's existing wheat.

Description

technical field [0001] The invention relates to a functional wheat biotechnology breeding method for increasing the selenium content of wheat, and belongs to the technical field of wheat breeding methods. Background technique [0002] Wheat is one of the most important rations in China, and the development of the wheat industry is directly related to national food security and social stability. Profound changes have taken place in the domestic resource environment, grain supply and demand pattern, and international trade situation. With a large population in China, ensuring national food security has always been a top priority in governance. Both the Central Economic Work Conference and the Central Rural Work Conference in 2013 put the issue of food security at the top of the priority list, and clearly stated that the national food security strategy is: "mainly based on China, based on domestic production, production capacity guaranteed, moderate imports, and scientific and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/87A01H5/10A01H6/46A01H1/02
CPCA01H1/02C12N15/87
Inventor 何登骥其他发明人请求不公开姓名
Owner 重庆汇鑫云强实业集团有限公司