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Method for establishing demodex-caused rosacea-like skin lesion animal model

A rosacea, animal model technology, applied in animal husbandry and other directions, can solve the problems of hindering the release of lamellar bodies, the inability of the stratum corneum penetration barrier function to be well repaired, and the inability to exert the barrier function, etc. The effect of low rate, anti-infection and strong adaptation rate

Pending Publication Date: 2019-09-27
罗洋
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Problems solved by technology

KLK5 can degrade corneous desmosomes and dissociate intercellular junctions, so that the stratum corneum cannot perform normal barrier function due to loss of integrity; KLK5 can also activate PAR2 and the downstream NF-κB pathway, and upregulate IL -8, the expression of inflammatory factors such as TSLP and TNF; at the same time, KLK5 plays a role in the expression process of PAR2 signaling pathway, hindering the release of lamellar bodies, so that the permeability barrier function of the stratum corneum cannot be well repaired

Method used

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  • Method for establishing demodex-caused rosacea-like skin lesion animal model
  • Method for establishing demodex-caused rosacea-like skin lesion animal model
  • Method for establishing demodex-caused rosacea-like skin lesion animal model

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Embodiment Construction

[0043] Below, in conjunction with accompanying drawing, technical solution of the present invention is described further:

[0044] A method for constructing an animal model of rosacea-like skin lesions caused by Demodex, the steps of which are:

[0045] Build animal models

[0046] 1. Selection and cultivation of Japanese big-eared rabbits

[0047] ① 24 healthy 12-week-old female common-grade Japanese big-eared rabbits [SCXK (Gan) 2015-0001] were selected, with a weight of 2.0-2.5 kg.

[0048] ② Grouping of animals: Japanese big-eared rabbits were fed for 1 week, randomly divided into experimental group and control group, with 12 animals in each group, and raised in separate cages in a clean animal room.

[0049] ③ Feeding conditions: temperature 23±2°C, humidity 54±10%, ventilated 2-3 times a day, each time lasting 1 hour, according to the natural life pattern of Japanese big-eared rabbits, given grass feed, so that they can drink water and eat freely , move freely, and fe...

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Abstract

The invention discloses a method for establishing a demodex-caused rosacea-like skin lesion animal model. The method includes the steps of cleaning and drying the skin of a facial-demodex-positive mite donor, squeezing and scraping the wings of a nose or a face to obtain sebum secretion, cleaning the squeezed secretion to prepare demodex protozoon suspension, and intradermally injecting the demodex protozoon suspension into a 4 cm*4 cm experimental area of the back of a Japanese rabbit. In order to solve the problems that the reactivity of an existing rabbit to the natural infection approach of human demodex is quite small and there is a lack of the demodex-caused rosacea-like skin lesion animal model, the Japanese rabbit species is adopted and demodex protozoon is inoculated intradermally, and the demodex-caused rosacea-like skin lesion animal model is established; the clinical expression and histopathological expression of erythema, papule and skin lesion highly similar to rosacea of demodex-positive people can be observed through the skin lesion of the Japanese rabbit, and the requirement of using the Japanese rabbit as the demodex-caused rosacea-like skin lesion animal model is met.

Description

technical field [0001] The invention relates to the technical field of animal models, in particular to a method for constructing an animal model of rosacea-like skin lesions caused by Demodex mite. Background technique [0002] Rosacea is a common chronic inflammatory skin disease involving facial blood vessels and pilosebaceous glands in patients. The clinical manifestations are transient or persistent erythema, telangiectasia, papules, and pustules mainly in the middle of the face. Some patients with a long course of disease may also have dark red plaques or even rhinophyma due to tissue hyperplasia. At present, the incidence of rosacea is increasing year by year, and the treatment lacks specific drugs. Because the disease damages the patient's facial beauty, it affects the patient's physical and mental health and quality of life. Some patients will suffer from depression, low self-esteem, cowardice, withdrawn personality, heavy mental burden, and loss of confidence in ...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K2207/12A01K2227/107A01K2267/03
Inventor 罗洋张剑虹吴岚曦周楠栾秀丽
Owner 罗洋
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