Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Composite amplifying system of SNP-DIP micro haplotype domain for medico-legal individual recognition based on NGS subtyping

A compound amplification and haplotype technology, applied in the research and development field of new forensic medicine, can solve the problem of low genetic polymorphism

Pending Publication Date: 2019-10-08
西安交通大学口腔医院
View PDF5 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

SNP and DIP genetic markers are mainly manifested as biallelic genetic variation. Compared with STR loci, the genetic polymorphism of these two genetic markers is relatively low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composite amplifying system of SNP-DIP micro haplotype domain for medico-legal individual recognition based on NGS subtyping
  • Composite amplifying system of SNP-DIP micro haplotype domain for medico-legal individual recognition based on NGS subtyping
  • Composite amplifying system of SNP-DIP micro haplotype domain for medico-legal individual recognition based on NGS subtyping

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The following takes a sample as an example to further describe the operation process of the present invention in detail. It should be pointed out that the following description is only an illustration of the technical solutions claimed in the present invention, and is not any limitation to these technical solutions. The protection scope of the present invention shall be determined by the contents described in the appended claims.

[0020] Reagent used in the present invention: ddH 2 O, 10×PCR buffer, 50nM Primer Mix, 2.5mM dNTP, 5U / µL Hot Start DNA polymerase, 100mM Mg 2+ and 2 µM barcode reagent.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of creative research and application of a medico-legal new technology, and particularly relates to and discloses a composite amplifying detection system of a micro haplotype domain consisting of SNP-DIP molecular genetic markers for medico-legal individual recognition. Based on SNP sites or DIP sites which are reported in the past, a research strategy of a microhaplotype domain is adopted, through a dbSNP library, whether flanking sequences (which are 100bp up and down) of the sites have other SNP or DIP heredity variations or not is consulted, and accordingto the combination type differences of heredity variations in each region, a molecular genetic marker similar to the micro haplotype domain is formed. The regions are deeply sequenced by a bibasic sequencing method, so that the simultaneous subtyping detection of a plurality of samples can be realized, and besides, the base sequence variation information of all SNP or DIP sites in each region canbe obtained. The 18 micro haplotype domains provided by the invention has higher accumulation individual recognition rate in East Asia groups, and can be used for application research of medico-legalindividual recognition.

Description

technical field [0001] The invention belongs to the field of research and development of new technologies in forensic medicine, and specifically relates to a compound amplification system of a micro-haplotype domain composed of single nucleotide polymorphism and deletion / insertion polymorphism molecular genetic markers and its second-generation sequencing-based analysis and detection methods. Background technique [0002] Single Nucleotide Polymorphism (SNP) is a sequence polymorphic molecular genetic marker formed by a single nucleotide variation in the genome; Deletion / Insertion Polymorphism (DIP) is an insertion in the genome Or length polymorphic molecular genetic markers formed by the deletion of DNA fragments of different lengths; these two genetic markers have the advantages of wide distribution in the genome, low mutation rate, and easy access to shorter amplicons. SNP and DIP genetic markers are mainly manifested as genetic variation of biallelic genes. Compared wi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/172C12Q2535/122C12Q2531/113
Inventor 朱波峰靳小业崔伟陈冲
Owner 西安交通大学口腔医院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com