Tobacco black shank resistance identification device and method

Abstract

The invention discloses a tobacco black shank resistance identification device and method and relates to the technical field of crop disease resistance identification. The method comprises the following steps of activation of black shank bacteria, preparation of an inducing spore suspension, infection of tobacco germplasm seedling samples to be identified, and statistics and identification of results. Hydroponic tobacco seedlings are adopted for resistance identification, and the identification process is also carried out in a hydroponic environment, so that the integrity of the roots of plants is maintained, experimental errors caused by the imbalance of root damage are prevented, and convenience is provided for observing the incidence condition and process. The device and the method areused for precisely controlling the inoculation intensity and improving the accuracy of identification of resistance to tobacco germplasm black shank.

Description

technical field [0001] The invention belongs to the technical field of crop disease resistance identification, and in particular relates to a tobacco black shank resistance identification device and identification method. Background technique [0002] Tobacco black shank ( Phytophthora parasitica var.nicotiane) is one of the most devastating diseases in tobacco production, and is the most important factor restricting the production of tobacco leaves in various tobacco areas in my country. tobacco. Tobacco black shank was first discovered in Java by Bredde Haan in 1896. In 1924, Cook observed the disease again in Puerto Rico. Since then, tobacco black shank has been reported successively in major tobacco-producing areas in the world, and now the disease has spread in temperate, subtropical and tropical smoking areas. In the 1940s, the disease area was expanded to the tobacco-producing provinces in the Yellow River Basin, the Yangtze River Basin and the Pearl River Basin, a...

AI Technical Summary

Problems solved by technology

[0004] At present, the problems of this technology are as follows: 1. The inoculation intensity cannot be accurately controlled through the inoculation of fungi, so the difference in the resistance of tobacco germplasm to black shank cannot be accurately identified; 2. Due to the low accuracy of environmental control, the onset time is too long; 3. , Can not accurately control the inoculation site, mycelium and spores may infect through the root and stem base

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