Serum miRNA detection kit related to coronary artery stenosis and application thereof

Abstract

The invention provides a whole-set kit capable of detecting miR-19a conveniently and quickly without special ordering of special primer sequences. The kit is used for detecting the expression level ofcirculating miR-19a in serum, and the expression level of miR-19a in plaque tissue of patients with coronary artery stenosis so as to research the expression of miR-19a in coronary artery stenosis and clinical significance of the miR-19a, thus providing a new idea for auxiliary diagnosis of coronary artery stenosis.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a detection kit of miR-19a and its application as a molecular marker in the diagnosis of coronary artery stenosis. Background technique [0002] Coronary artery stenosis refers to coronary artery stenosis caused by coronary atherosclerosis, which seriously threatens human health diseases including: coronary heart disease, stroke, and peripheral vascular disease. These diseases account for the vast majority of cardiovascular disease morbidity and mortality, and more and more evidence shows that coronary atherosclerosis is the pathological basis of coronary artery stenosis. Atherosclerosis is a chronic inflammatory disease characterized by the accumulation of massive lipids and inflammatory cells in the arterial walls. At present, it is believed that this process starts with the accumulation of low-density lipoprotein in the intima. When the arterial wall is ...

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Problems solved by technology

[0004] Patent 2010800635211 discloses a biomarker for the diagnosis and classification of cardiovascular diseases, which uses a variety of prediction models and AUC, NRI and CNRI features to predict a variety of miRNAs in predicting cardiovascular diseases through training models, but It does not disclose the composition of the kit of the present application, the expression levels of specific miRNAs in plasma and adjacent tissues, and the specific relationship between miRNA expression levels and coronary artery stenosis symptoms

[0005] At present, the detection methods of miRNA research at home and abroad are mainly chip technology, sequencing technology and real-time fluorescent quantitative PCR reaction. Large-scale screening; sequencing technology can accurately confirm the type of miRNA by detecting the base type, but it is also expensive, time-consuming, and cannot be accurately quantified, so it is not adopted as a routine method for miRNA detection; real-time fluorescence quantification PCR technology has the advantages of simple and convenient operation, relatively quantitative miRNA content, and low cost, making it a routine method for miRNA detection. However, most of the PCR kits for miRNA detection on the market require additional Formulate special miRNA reverse transcription primers and upstream and downstream primers for quantitative PCR detection, which makes detection inconvenient

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