Extraction and separation method of akebia trifoliata pericarp polysaccharide

Abstract

The invention discloses an extraction and separation method of akebia trifoliata pericarp polysaccharide. Fruits of the akebia trifoliata are washed and peeled, and the pericarp is washed with distilled water, placed in a baking oven for drying and crushed to obtain akebia trifoliata pericarp powder; the akebia trifoliata pericarp powder is degreased by petroleum ether, molecular sugar-removed byethanol, soaked with water, extracted by ultrasonic wave and centrifuged after the extraction, and liquid after the centrifugation is collected to obtain an extract; the extract is concentrated, ethanol is added for alcohol precipitation, an alcohol precipitate is crude total polysaccharide, and protein removal, alcohol precipitation, washing, dialysis and drying are carried out to obtain refinedtotal polysaccharide; and the polysaccharide is freeze-drying and crushed to obtain the akebia trifoliata pericarp polysaccharide. The extraction and separation method of the invention can quickly extract the polysaccharide in the akebia trifoliata pericarp product.

Description

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AI Technical Summary

Benefits of technology

This patented innovation improves upon existing methods by providing faster and more effective ways to extract specific compounds while minimizing harm or contamination caused when other materials are mixed together. These techniques involve both chemical reactions that break down certain substances into smaller molecules like sugars (Acebium) and proteins called okadaic acid, resulting in better purity products compared to previous processes.

Problems solved by technology

This patented technical problem addressed by this patents relates to extracting specific types of polygulan gums that can improve their effectiveness for various applications like medicine, healthcare products, cosmetic ingredients, biological samples, environmental remedies, fertilizers, pesticide compositions, antimicrobial agents, diabetes treatment drugs, cancer therapy treatments, hyperlipemia prevention, radiation protection, virus resistance, aging control, skin care, hair growth promoters, nutritional supplementation, dentistry, veterinary medicines, among others.