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Inoculation method of apple ring rot germs

A technology for apple ringworm and ringworm bacteria, applied in the agricultural field, can solve the problems of unstable effect, affecting biological characteristics, pathogenic mechanism and drug toxicity research, and less induced spore production

Inactive Publication Date: 2019-10-29
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods take a long time to induce, and require certain equipment conditions, and the amount of induced sporulation is small, and the effect is unstable, so it is difficult to meet the needs of large-scale inoculation experiments.
This affects the research on its biological characteristics, pathogenic mechanism and drug toxicity, which in turn affects the research and development of the disease prevention and control measures

Method used

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  • Inoculation method of apple ring rot germs
  • Inoculation method of apple ring rot germs
  • Inoculation method of apple ring rot germs

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Embodiment Construction

[0023] The present invention will be described in detail below in combination with specific embodiments.

[0024] Apple ring bacteria solution obtains:

[0025] Bacterial liquid shaking culture: In the ultra-clean workbench, take 50mL of PDA liquid medium and add it to a high-temperature sterilized 100mL Erlenmeyer flask, and use sterile pointed tweezers to pick a little from the apple ringworm cultured on the plate Put the mycelia into the Erlenmeyer flask, seal it with a sealing film, place it in a shaker at 28°C and 200rpm and cultivate it for 3 days.

[0026] In the ultra-clean workbench, take 30mL PDA liquid medium and add it to a 50mL centrifuge tube, and then clamp two or three mycelia balls from the culture in the Erlenmeyer flask and put them into a 50mL centrifuge tube;

[0027] Take a large beaker, fill it up with ice cubes, insert the above 50mL centrifuge tube into the ice cube and fix it in the beaker;

[0028] Put the beaker into the soundproof box of the ultr...

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Abstract

The invention discloses an inoculation method of apple ring rot germs. The inoculation method comprises the steps of obtaining an apple ring rot germ solution, inoculating apple fruits in the germ solution, and finally inoculating branches. According to a formula, a PDA solution nutrient medium is prepared from the following components: 200g of a potato, 20g of glucose and 1000ml of distilled water. The inoculation method has the beneficial effects that a method that a fungus cake inoculates apple ring rot in majority of studies at present can be supplemented or replaced, large-quantity and accurate inoculation can be achieved, and the inoculation method has the characteristics of being simple and efficient.

Description

technical field [0001] The invention belongs to the technical field of agriculture and relates to an inoculation method of apple ringworm. Background technique [0002] At present, apple ring disease has become one of the important factors that seriously restrict the healthy and sustainable development of my country's apple industry. In 2008, a survey of 88 orchards in 7 major apple-producing provinces and cities in my country, including Shandong, showed that the overall incidence of apple ring spot was 77.6%, and the incidence in Shandong and Henan provinces was as high as 100%. Ringworm fungus infects the branches, forming tumors, rough bark and dry rot, which seriously affects the tree vigor and eventually leads to the death of the tree body or branches; ringworm fungus infects the fruit, and in severe cases, the fruit rots, resulting in serious yield and quality decline. According to reports, in normal years, the diseased fruit rate of fruits harvested in conventional ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G7/06C12Q1/18C12Q1/04C12R1/645
CPCC12N1/14A01G7/06C12Q1/18C12Q1/04G01N2333/37
Inventor 黄永红
Owner QINGDAO AGRI UNIV