A strain of Paleobacterium intermedia b522 and its application

A technology of Paleobacterium intermedia, cgmccno.17652, which is applied in the direction of bacteria, biological water/sewage treatment, water treatment parameter control, etc. It can solve the problems of human and animal health threats, increased treatment difficulty, refractory degradation, etc., and achieve wide temperature adaptability, Obvious removal effect, good degradation effect

Active Publication Date: 2021-04-13
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, large-scale breeding can greatly shorten the growth cycle of livestock and poultry, increase production, and reduce costs. On the other hand, due to the large-scale accumulation of pollutants, the difficulty of treatment increases, so the environmental pollution is not to be underestimated. watch for
Livestock wastewater has high concentration of suspended solids, COD cr High content, BOD 5 High organic phosphorus content, high ammonia nitrogen content, difficult to transport, and difficult to degrade. In addition, livestock and poultry wastewater contains a large number of pathogenic microorganisms, parasite eggs, and residual heavy metal elements and antibiotics. The air, water, and soil environment cause serious harm, destroy the natural ecological balance, and pose a great threat to human and animal health

Method used

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  • A strain of Paleobacterium intermedia b522 and its application
  • A strain of Paleobacterium intermedia b522 and its application
  • A strain of Paleobacterium intermedia b522 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Isolation and identification of strains:

[0016] Mix fresh pig urine collected from a pig farm in Daxing, Beijing, with tap water at a ratio of 1:4, add fresh pig manure with a mass fraction of 5% and mix evenly, after standing for 24 hours, take the upper layer of liquid as pig breeding sewage for later use .

[0017] The above sewage was randomly divided into groups and naturally fermented on a shaking table at 30°C and 200rpm for 3 days, and then samples were taken to detect changes in the content of COD, ammonia nitrogen and total phosphorus. The group with the most obvious decrease in pollutant content was selected and 5 mL of fermentation broth was inoculated into the enrichment medium. Cultivate at 30°C, 200rpm shaker for 24h, then continue to inoculate 5mL culture solution into new enrichment medium, and culture repeatedly for 5 generations.

[0018] Enrichment medium: the above-mentioned pig breeding sewage was sterilized at 121°C for 30 minutes as an enrichm...

Embodiment 2

[0031] Degradation of ammonia nitrogen sewage by bacterial strains:

[0032] LB medium: 5 g of yeast extract, 10 g of tryptone, 10 g of sodium chloride, and 1 L of distilled water.

[0033] Ammonia nitrogen sewage: glucose 0.62g, sodium acetate 2g, ammonium sulfate 1g, Vickers salt 50mL, distilled water 1L, pH 7.5. Vickers salt: 5g of dipotassium hydrogen phosphate, 2.5g of magnesium sulfate heptahydrate, 2.5g of sodium chloride, 0.05g of ferrous sulfate heptahydrate, 0.05g of manganese sulfate tetrahydrate, 1L of distilled water.

[0034] The above medium was sterilized at 121°C for 30 min before use.

[0035] Pick a single bacterial colony from the plate and put it into LB liquid, and cultivate it at 30°C for 24 hours to activate it. Under sterile conditions, add the B522 cells that have been centrifuged to remove the culture medium into the ammonia nitrogen sewage at a volume fraction of 5%, and set the culture condition to 30 ℃, 200rpm, samples were taken at 0d, 1d, 4d, ...

Embodiment 3

[0039] Degradation of the strains on livestock and poultry breeding sewage:

[0040] The pretreatment method of livestock and poultry breeding sewage is to mix fresh pig urine collected from a pig farm in Daxing, Beijing, with tap water at a ratio of 1:4, then add fresh pig manure with a mass fraction of 5%, stir evenly, and let stand After 24 hours, the upper layer liquid was taken as laboratory livestock and poultry breeding sewage for later use.

[0041]Add 0.5% molasses to the above-mentioned livestock and poultry breeding sewage as an additional carbon source, and use it for the degradation experiment of the strain after high temperature and high pressure sterilization. Pick the B522 single colony on the plate into the LB liquid, and cultivate it at 30°C for 24 hours to activate it. Under sterile conditions, insert the B522 cells after centrifugation to remove the culture medium into the livestock and poultry sewage at a volume fraction of 5%, and set The culture conditi...

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Abstract

The invention discloses a strain of Ochrobactrum intermedium B522 that synergistically degrades COD and ammonia nitrogen in livestock and poultry breeding sewage and its application. The bacterium has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on April 29, 2019 , referred to as CGMCC, the address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No.17652. The strain has a wide range of suitable growth temperatures, and can proliferate well between 20-45°C (OD 600 >1.0). Its optimum growth pH is between 6.0‑8.5 (OD 600 >1.0). The aquaculture wastewater collected from actual pig farms in the laboratory had initial COD, ammonia nitrogen, and total phosphorus contents of 7600 mg / L, 204 mg / L, and 19.75 mg / L, respectively. After 3 days of aerobic treatment with strain B522, the experimental results showed that the The bacteria achieved a large number of proliferation in the first 24 hours, and the proliferation speed of the bacteria slowed down within 24-72 hours until reaching the maximum concentration of bacteria. In this process, the COD degradation rate in the aquaculture wastewater was 66%, the ammonia nitrogen degradation rate was 84%, and the total phosphorus removal rate was 40%. Therefore, the strain has a good degradation effect on COD, ammonia nitrogen and total phosphorus, so it has great potential in the microbial treatment of livestock and poultry wastewater, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to the technical field of microbial wastewater treatment. Background technique [0002] In recent years, with the continuous adjustment of my country's agricultural industrial structure, the scale and intensification of the livestock and poultry breeding industry have continued to increase. On the one hand, large-scale breeding can greatly shorten the growth cycle of livestock and poultry, increase production, and reduce costs. On the other hand, due to the large-scale accumulation of pollutants, the difficulty of treatment increases, so the environmental pollution is not to be underestimated. watch for. Livestock wastewater has high concentration of suspended solids, COD cr High content, BOD 5 High organic phosphorus content, high ammonia nitrogen content, difficult to transport, and difficult to degrade. In addition, livestock and poultry wastewater contains a large numb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/20
CPCC02F3/34C02F2103/20C02F2209/08C02F2209/14C12N1/20C12N1/205C12R2001/01
Inventor 李少杰曹先贺孙宪昀张振颖
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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