A strain of Paleobacterium intermedia b522 and its application
A technology of Paleobacterium intermedia, cgmccno.17652, which is applied in the direction of bacteria, biological water/sewage treatment, water treatment parameter control, etc. It can solve the problems of human and animal health threats, increased treatment difficulty, refractory degradation, etc., and achieve wide temperature adaptability, Obvious removal effect, good degradation effect
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Embodiment 1
[0015] Isolation and identification of strains:
[0016] Mix fresh pig urine collected from a pig farm in Daxing, Beijing, with tap water at a ratio of 1:4, add fresh pig manure with a mass fraction of 5% and mix evenly, after standing for 24 hours, take the upper layer of liquid as pig breeding sewage for later use .
[0017] The above sewage was randomly divided into groups and naturally fermented on a shaking table at 30°C and 200rpm for 3 days, and then samples were taken to detect changes in the content of COD, ammonia nitrogen and total phosphorus. The group with the most obvious decrease in pollutant content was selected and 5 mL of fermentation broth was inoculated into the enrichment medium. Cultivate at 30°C, 200rpm shaker for 24h, then continue to inoculate 5mL culture solution into new enrichment medium, and culture repeatedly for 5 generations.
[0018] Enrichment medium: the above-mentioned pig breeding sewage was sterilized at 121°C for 30 minutes as an enrichm...
Embodiment 2
[0031] Degradation of ammonia nitrogen sewage by bacterial strains:
[0032] LB medium: 5 g of yeast extract, 10 g of tryptone, 10 g of sodium chloride, and 1 L of distilled water.
[0033] Ammonia nitrogen sewage: glucose 0.62g, sodium acetate 2g, ammonium sulfate 1g, Vickers salt 50mL, distilled water 1L, pH 7.5. Vickers salt: 5g of dipotassium hydrogen phosphate, 2.5g of magnesium sulfate heptahydrate, 2.5g of sodium chloride, 0.05g of ferrous sulfate heptahydrate, 0.05g of manganese sulfate tetrahydrate, 1L of distilled water.
[0034] The above medium was sterilized at 121°C for 30 min before use.
[0035] Pick a single bacterial colony from the plate and put it into LB liquid, and cultivate it at 30°C for 24 hours to activate it. Under sterile conditions, add the B522 cells that have been centrifuged to remove the culture medium into the ammonia nitrogen sewage at a volume fraction of 5%, and set the culture condition to 30 ℃, 200rpm, samples were taken at 0d, 1d, 4d, ...
Embodiment 3
[0039] Degradation of the strains on livestock and poultry breeding sewage:
[0040] The pretreatment method of livestock and poultry breeding sewage is to mix fresh pig urine collected from a pig farm in Daxing, Beijing, with tap water at a ratio of 1:4, then add fresh pig manure with a mass fraction of 5%, stir evenly, and let stand After 24 hours, the upper layer liquid was taken as laboratory livestock and poultry breeding sewage for later use.
[0041]Add 0.5% molasses to the above-mentioned livestock and poultry breeding sewage as an additional carbon source, and use it for the degradation experiment of the strain after high temperature and high pressure sterilization. Pick the B522 single colony on the plate into the LB liquid, and cultivate it at 30°C for 24 hours to activate it. Under sterile conditions, insert the B522 cells after centrifugation to remove the culture medium into the livestock and poultry sewage at a volume fraction of 5%, and set The culture conditi...
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