Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel baculovirus expression vector

A baculovirus and expression vector technology, applied in the field of new baculovirus expression vector, can solve the problems of unsatisfactory yield and high production cost of baculovirus expression system, and achieve excellent high-yield characteristics, excellent production traits, protein The effect of increased expression levels

Active Publication Date: 2019-10-29
NORTHWEST A & F UNIV
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, compared with the prokaryotic expression system (Escherichia coli, Bacillus subtilis) and yeast expression system commonly used in industry, the output of the baculovirus expression vector system is not satisfactory, which leads to the production cost of the baculovirus expression system. high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel baculovirus expression vector
  • Novel baculovirus expression vector
  • Novel baculovirus expression vector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Knockout of Ac84-87 gene

[0023] Using the primers (SEQ ID NO: 1 and SEQ ID NO: 2) with the upstream and downstream 50bp homology arms of the Ac84-87 DNA fragment, and using the pTriEx1.1 plasmid as a template, the ampicillin resistance gene fragment was amplified to obtain a 1023bp The PCR product (SEQID NO:3).

[0024] The obtained PCR product was transformed into E. coli strain HS996 with RedET plasmid and Bacmid by electroporation, induced by arabinose to produce recombinase, and the recombined E. coli was screened on an ampicillin resistance plate to obtain positive clones. At this point the Ac84-87 DNA fragment has been replaced by the ampicillin resistance gene fragment. Bacmid was extracted from Escherichia coli and named BacmidΔAc84-87 after sequencing and identification (see the appendix for the knockout strategy figure 1 ).

[0025] BacmidΔAc84-87 was used for subsequent experiments after plasmid extraction and Bsu36I restriction endonuclease digestion...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a novel baculovirus expression vector, and relates to the technical field of biology. The novel baculovirus expression vector is obtained by simultaneously knocking out four consecutive non-essential genes Ac84, Ac85, Ac86 and Ac87 on a baculovirus expression vector. The novel baculovirus expression vector increases the expression level of foreign proteins by 80%, and meanwhile, the proliferation characteristics of the virus are not affected. The increased expression yield can significantly reduce the production cost of enterprises. The baculovirus expression vector canbe used in the field of biological products industry, especially in the field of subunit vaccine industry.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a novel baculovirus expression vector. Background technique [0002] Baculovirus is a double-stranded DNA virus that specifically infects arthropods. Autographa californica nucleopolyhedrovirus (AcMNPV) is the model species of baculovirus. Since Smith GE et al first expressed human β-interferon gene in insect cells with baculovirus in 1983 (Mol Cell Biol.1983; 3:2156-65.), due to its low cost, high yield, and various translation Post-modification system and other advantages, the baculovirus expression vector system is widely used in scientific research and production, especially in the fields of biological products and vaccine industry. [0003] However, compared with the prokaryotic expression system (Escherichia coli, Bacillus subtilis) and yeast expression system commonly used in industry, the output of the baculovirus expression vector system is not satisfactory, which leads to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/866C07K14/435
CPCC12N15/86C07K14/43595C12N2710/14143C12N2710/14134
Inventor 陈红英张潇月赵凯霞
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com