Analysis system

Abstract

The present invention relates to an analysis system. The system is applicable to automatic rapid analysis and detection of high-fat soluble components. According to the system provided by the presentinvention, on-line pretreatment and separation of a sample containing high-fat soluble components is performed on a to-be-tested sample by using a multi-dimensional chromatography system; the multi-dimensional chromatography system comprises a supercritical chromatography portion and a reverse phase liquid chromatography portion which are sequentially connected; and the reverse phase liquid chromatography portion comprises one or more reverse phase liquid chromatography columns, and the supercritical chromatography portion comprises a supercritical mobile phase, a modifier and a supercriticalpacking column.

Description

technical field [0001] The invention relates to an analysis system, which is suitable for separating highly fat-soluble components from oil or biological samples, especially for separating vitamin components from oil or biological samples, and belongs to the field of separation of natural substances. Background technique [0002] The separation of highly fat-soluble components from some oils or biological samples has always been a research hotspot, and various separation and extraction equipment and methods have been studied in the prior art. [0003] Vitamin components, especially the isolation of vitamin D components, have also been extensively attempted. Vitamin D 2 (also known as ergocalciferol) and vitamin D 3 (cholecalciferol) are fat-soluble vitamins that play a very important role in calcium metabolism. Vitamin D also affects other metabolic pathways. A lack of vitamin D can lead to serious diseases such as rickets and weakened bones. Often, vitamin D supplement...

AI Technical Summary

Problems solved by technology

[0016] For the high fat-soluble components in the above-mentioned prior art, especially the method and analysis system for extracting high fat-soluble components from complex systems such as oil or biological samples, the pretreatment process is complicated, the solvent consumption is large, and each chromatographic To solve the problems of lack of convenience in switching between analyzes, the present invention adopts a multi-dimensional chromatographic system to provide a method and analysis system for rapid analysis of high fat-soluble components, especially vitamin D in oil or biological samples, which uses supercritical chromatography and The combination of reversed-phase liquid chromatography can realize simple and rapid separation of high fat-soluble components contained in oil or biological samples, especially vitamin D, and then use detection instruments to detect and analyze the separated vitamin D

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