Construction method of electrochemical immunosensor based on Au@Co-MOFs composite material

An immune sensor and composite material technology, which is applied in the direction of scientific instruments, analytical materials, material inspection products, etc., can solve the problems that the accuracy cannot meet the requirements, achieve good accuracy, enhance the measured electrical signal, and large specific surface area Effect

Active Publication Date: 2019-11-05
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is not accurate enough for ultrasensitive detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) add the mixed acid (fuming nitric acid: sulfuric acid=1: 1.2 volume ratio) that is mixed by the concentrated sulfuric acid and fuming nitric acid in reaction vessel, add the terephthalic acid (mixed acid: terephthalic acid) of 12g under stirring condition at room temperature Phthalic acid = 1.01:1 molar ratio), the feeding time is 27min; heat to 66°C within 60min, keep warm for 75min, continue to heat up to 80°C, then control the temperature to exceed 85°C, keep warm for 3.5h, and cool the obtained reactant , poured into crushed ice water, filtered the precipitate, washed with water until neutral, and recrystallized in water to obtain 9.3g of 2-nitroterephthalic acid for subsequent use;

[0032] 2) Add 28ml concentrated hydrochloric acid to the reaction vessel, add 13g SnCl under stirring condition 2 2H 2 O, after all dissolved, add 7g of 2-nitroterephthalic acid; heat at 98°C within 35min, react for 2h, cool to room temperature, filter, wash the filter cake with di...

Embodiment 2

[0038] 1) Add the mixed acid (fuming nitric acid: sulfuric acid=1: 1.2 volume ratio) that is mixed by the concentrated sulfuric acid and fuming nitric acid in reaction vessel, add the terephthalic acid (mixed acid: terephthalic acid) of 15g under stirring condition at room temperature Phthalic acid=1.01:1 molar ratio), the feeding time is 35min; heat to 70°C within 65min, keep warm for 60min, continue to heat up to 81°C, then control the temperature to exceed 85°C, keep warm for 3h, and cool the obtained reactant, Pour into crushed ice water, filter the precipitate, wash with water until neutral, and recrystallize in water to obtain 10.8g of 2-nitroterephthalic acid, which is set aside;

[0039] 2) Add 35ml concentrated hydrochloric acid to the reaction vessel, add 15g SnCl under stirring condition 2 2H 2 O, after all dissolved, add 8g of 2-nitroterephthalic acid; heat at 100°C within 40min, react for 2h, cool to room temperature, filter, wash the filter cake with dilute hydr...

Embodiment 3

[0045] 1) add the mixed acid (fuming nitric acid: sulfuric acid=1: 1.2 volume ratio) that is mixed by the concentrated sulfuric acid and fuming nitric acid in reaction vessel, add the terephthalic acid (mixed acid: terephthalic acid) of 13g under stirring condition at room temperature Phthalic acid=1.01:1 molar ratio), the feeding time is 35min; heat to 70°C within 65min, keep warm for 60min, continue to heat up to 81°C, then control the temperature to exceed 85°C, keep warm for 3h, and cool the obtained reactant, Pour into crushed ice water, filter the precipitate, wash with water until neutral, and recrystallize in water to obtain 10.2g of 2-nitroterephthalic acid, which is set aside;

[0046] 2) Add 35ml concentrated hydrochloric acid to the reaction vessel, add 14g SnCl under stirring condition 2 2H 2 O, after all dissolved, add 8g of 2-nitroterephthalic acid; heat at 100°C within 40min, react for 2h, cool to room temperature, filter, wash the filter cake with dilute hydr...

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PUM

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Abstract

The invention relates to the field of electrochemical immunosensors, and discloses a construction method of an electrochemical immunosensor based on a Au@Co-MOFs composite material. The method comprises the steps of 1) preparation of 2-nitro terephthalic acid; 2) preparation of 2-amino terephthalic acid; 3) preparation of a Co-MOFs material; 4) preparation of the Au@Co-MOFs composite material; 5)construction of the electrochemical immunosensor based on the Au@Co-MOFs composite material. By means of the construction method of the electrochemical immunosensor based on the Au@Co-MOFs composite material, the electrochemical immunosensor based on the Au@Co-MOFs composite material can be constructed, can be used for ultrasensitive detection, is higher in accuracy and sensitivity, and has quiteimportant practical significance in the field of electrochemical immunosensors of China.

Description

technical field [0001] The invention relates to the field of electrochemical immunosensors, in particular to a method for constructing electrochemical immunosensors based on Au@Co-MOFs composite materials. Background technique [0002] MOFs is an abbreviation for metal organic framework compounds. It is a kind of crystalline porous material with a periodic network structure formed by the interconnection of inorganic metal centers (metal ions or metal clusters) and bridging organic ligands through self-assembly. MOFs is an organic-inorganic hybrid material, also known as a coordination polymer, which is different from both inorganic porous materials and general organic complexes. This combination of the rigidity of inorganic materials and the flexibility of organic materials presents a huge development potential and attractive development prospects in modern materials research. [0003] Electrochemical immunosensors have the characteristics of high sensitivity, simple equip...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N27/327
CPCG01N33/53G01N27/308G01N27/3278
Inventor 彭志勤何宇杰刘珉琦黄诗莹万军民
Owner ZHEJIANG SCI-TECH UNIV
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