Immunogenic composition containing rabbit staphylococcus antigen, preparation method and application thereof

A staphylococcal and immunogenic technology, applied in the field of veterinary biological products, can solve the problems of vaccine strain failure, storage space occupation, and reduced protection rate, and achieve the effects of easy storage, high porosity, and guaranteed firmness

Active Publication Date: 2022-07-12
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the vaccines in the prior art are injections and freeze-dried powders, which are large in size and take up space for storage, and it is easy to cause the failure of the vaccine strain during storage, resulting in a reduction in the protection rate

Method used

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  • Immunogenic composition containing rabbit staphylococcus antigen, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Preparation of Rabbit Staphylococcus Antigen Strain

[0030] 1) The intestinal bacteria and respiratory bacteria isolated and cultured from the liver and lung of the dead rabbit infected with Staphylococcus aureus were used for recovery as strains. After passing the pure bacteria inspection, the two strains were inoculated into ordinary broth medium respectively. Incubate at 37°C for 20h to prepare bacterial liquid with a predetermined concentration;

[0031] 2) Using turbidimetry and ultraviolet spectrophotometry to determine the concentration of respiratory bacteria to be 300 million / mL, add formaldehyde solution according to 0.3% of the bacterial liquid volume, and inactivate at 37°C for 20h; determine the concentration of intestinal bacteria to be 1 billion / mL mL, first bathed at 100°C for 2.5h, then added 0.3% formaldehyde solution and inactivated at 37°C for 20h;

[0032] 3) Inoculate the intestinal bacteria and respiratory bacteria treated in step 2) on a co...

Embodiment 2

[0043] This embodiment is basically the same as Embodiment 1, except that:

[0044] The dispersing carrier in this embodiment is chitosan nanoparticles loaded with disperse protein B, which can promote the overall diffusion of the biofilm and is conducive to the dispersion and release of the antigenic strains of Staphylococcus rabbits.

[0045] The preparation method of the dispersion carrier is as follows: adding dispersin B solution to a chitosan acetic acid solution with a concentration of 4.0 mg / ml until the final concentration of dispersin B is 2.0 mg / ml, at 30-35° C. and under the condition of magnetic stirring Mix well, let stand at 25°C for 22h, and add phosphate ester with a final concentration of 1.5mg / ml under the condition of low-speed magnetic stirring at 200rpm to activate the chitosan nanoparticle, which is convenient for loading disperse protein B, and keep stirring for 35min. The obtained chitosan nanoparticles loaded with disperse protein B have the character...

Embodiment 3

[0051] This embodiment is basically the same as Embodiment 2, except that:

[0052] The water-based printing adjuvant in this example adopts the commercially available Montanide TM Gel's aqueous vaccine adjuvant.

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Abstract

The invention discloses an immunogenic composition containing rabbit staphylococcus antigen, a preparation method and application thereof. The immunogenic composition comprises a mixture of rabbit staphylococcus antigen, dispersion carrier and aqueous printing adjuvant as a printing spray liquid , and the freeze-dried protective powder is used as the coating powder, and the printing spraying liquid is repeatedly sprayed on each layer of the coating powder by using the 3DP printing technology to obtain a three-dimensional structure of the immunogenic composition containing the rabbit staphylococcus antigen. The immunogenic composition prepared by the method of the invention has good immunogenic activity and immune protection, can be effectively used for the prevention and treatment of rabbit Staphylococcus aureus, the immune protection rate is as high as 100%, and the vaccine has good stability and is not easy to be inactivated.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and particularly relates to an immunogenic composition containing a rabbit staphylococcus antigen and a preparation method and application thereof. Background technique [0002] Humans are the natural reservoir hosts for S. aureus. Healthy individuals can be persistently (10-35%), intermittent (20-75%), or non-carrier (5-70%) colonized on the skin, nostrils, and throat with S. aureus without associated disease. Huge human reservoir hosts increase the chances of adapted pathogenic clonal types evolving and spreading. Invasive staphylococcal infections from the Gram-positive cocci Staphylococcus aureus and S. epidermidis are of particular concern as they are an increasing global public health problem. Specifically, Staphylococcus aureus is responsible for the majority of hospital-acquired (nosocomial) infections, and its prevalence in community-onset infections has increase...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/085A61K9/20A61K47/42A61K47/36A61P31/04
CPCA61K39/085A61K9/2063A61K9/205A61K9/2054A61P31/04A61K2039/552Y02A50/30
Inventor 孙晴刘言娟张桂芝葛艳艳王美欣
Owner LINYI UNIVERSITY
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