Tissue Culture and Rapid Propagation Method of Alpine Rhododendron Xiaqiuluo
A Rhododendron and Alpine technology, applied in the field of tissue culture and fast propagation of Alpine Rhododendron Xiaqiuluo, can solve the problems of high price of finished flowers, low survival rate, few thick leaves and buds, etc., to promote growth and development, improve survival rate, and transplant survival. The effect of rate increase
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[0020] The preparation method of the Rhododendron xiaqiuluo symbiotic bacterial liquid is as follows:
[0021] Step 41: Dissolve 1g of the rhizosphere soil of Rhododendron xiaqiuluo in 15mL of sterile water, and shake it on a shaker at 100-150rpm for 1-2h to obtain the rhizosphere soil extract, and then spread the rhizosphere soil extract On the ampicillin PDA plate, culture at 20-28°C for 24 hours, pick colonies, dissolve in 1mL sterile water, shake well, and store at 4°C.
[0022] In the above-mentioned alpine Rhododendron Xia Qiuluo tissue culture rapid propagation method, by adding the symbiotic bacteria liquid to the rooting and strong seedling medium in the later stage of the alpine Rhododendron Xia Qiuluo rooting and strong seedling cultivation, the symbiotic bacteria liquid is separated from the rhizosphere soil of the alpine Rhododendron Xia Qiuluo It is prepared by culturing, so that Rhododendron xiaqiuluo can use the symbiotic effect of symbiotic bacteria after root...
Embodiment 1
[0032] A kind of alpine Rhododendron xiaqiuluo tissue culture rapid propagation method, comprises the following steps:
[0033] Step 1: intercepting the stem tip of Rhododendron xiaqiuluo for disinfection to obtain aseptic explants;
[0034] Step 2: Implant the clustered bud induction medium to the sterile explants, carry out the clustered bud induction culture, and obtain the clustered buds; the clustered bud induction medium is 1 / 4MS+ZT 2.0mg / L+IAA 0.5mg / L ;
[0035] Step 3: Intercept the stem nodes of the clustered buds obtained in step 2, and lay flat and transplant them to the subculture medium respectively, and carry out subculture. After the subculture, adventitious buds grow at each stem node; the subculture The period of culture is 30 days, and the subculture is 3 times in total; the subculture medium is 1 / 4MS+ZT1.0mg / L+IAA0.5mg / L+GA31.0mg / L;
[0036] Step 4: Transplant the stem nodes with adventitious buds to the medium for rooting and strong seedlings. After culti...
Embodiment 2
[0042] A kind of alpine Rhododendron xiaqiuluo tissue culture rapid propagation method, comprises the following steps:
[0043] Step 1: intercepting the stem tip of Rhododendron xiaqiuluo for disinfection to obtain aseptic explants;
[0044] Step 2: Implant the clustered bud induction medium to the sterile explants, carry out the clustered bud induction culture, and obtain the clustered buds; the clustered bud induction medium is 1 / 4MS+ZT2.0mg / L+IAA 0.5mg / L ;
[0045]Step 3: Intercept the stem nodes of the clustered buds obtained in step 2, and lay flat and transplant them to the subculture medium respectively, and carry out subculture. After the subculture, adventitious buds grow at each stem node; the subculture The period of cultivation is 30 days, and subculture is carried out twice; the subculture medium is 1 / 4MS+ZT0.5mg / L+IAA0.5mg / L+GA31.0mg / L;
[0046] Step 4: Transplant the stem nodes with adventitious buds to the medium for rooting and strengthening seedlings. After...
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