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Breeding method of a light green leaf three-line sterile line, indica-japonica intermediary restorer line and indica-japonica inter-subspecies hybrid rice

A three-line sterile line, restorer line technology, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of greedy young and late maturity to chromatic aberration, etc., and achieve the effect of improving greedy young and late maturity

Active Publication Date: 2021-01-08
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method involves the use of light green leafy rice as a marker, and does not involve solving the problems of late maturation and color difference in later stages of hybrid rice between indica and japonica subspecies

Method used

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  • Breeding method of a light green leaf three-line sterile line, indica-japonica intermediary restorer line and indica-japonica inter-subspecies hybrid rice
  • Breeding method of a light green leaf three-line sterile line, indica-japonica intermediary restorer line and indica-japonica inter-subspecies hybrid rice
  • Breeding method of a light green leaf three-line sterile line, indica-japonica intermediary restorer line and indica-japonica inter-subspecies hybrid rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Breeding of the three-line japonica CMS line Chunjiang 24A with light green leaves: the maintainer line Chunjiang 26B of non-light green leaf japonica rice with excellent agronomic traits was selected as the female parent, and crossed with Chunjiang 19B, a japonica rice material with light green leaves and excellent comprehensive agronomic traits, in the F2 generation A single plant with light green leaves with excellent comprehensive agronomic traits was selected and self-crossed for 4 generations. The genetics were basically stable, and Chunjiang 24B was obtained. It was testcrossed with the Dian I-type CMS line Chunjiang 16A and backcrossed continuously for 5 generations. The sterility was stable and flowering was selected. Chunjiang 24A is a japonica three-line CMS line with good habit, high outcross seed setting rate, excellent plant and leaf shape, and light green leaf color.

[0061] Table 1. Comparison of agronomic characters between Chunjiang 24A and Chunjiang 1...

Embodiment 2

[0065] Breeding of the three-line japonica CMS line Chunjiang 28A with light green leaves: the light green leaf japonica rice material Chunjiang 19B with excellent comprehensive agronomic traits was selected as the female parent, crossed with the japonica rice maintainer Chunjiang 028, and the hybrid progeny F2 was selected with excellent comprehensive agronomic traits After 3 generations of self-crossing with the Dian I-type CMS line Chunjiang 16A and continuous backcrossing for 5 generations, the selected plants have stable sterility, good flowering habit, high outcrossing seed-setting rate, and excellent plant and leaf morphology. , a japonica three-line male sterile line Chunjiang 28A with light green leaves.

[0066] Table 2. Comparison of agronomic characters between Chunjiang 28A and Chunjiang 16A

[0067]

[0068] In the above Examples 1 and 2, the light green leaf japonica rice maintainer Chunjiang 19B was used as the parent, either as the female parent or as the m...

Embodiment 3

[0071] Breeding of light green leaf indica-japonica intermediate restorer line CH101: the non-light green leaf indica-japonica intermediate restorer line CH50 with excellent agronomic traits was selected as the female parent, and crossed with the light green leaf indica-japonica intermediate restorer line C52 with excellent comprehensive agronomic traits , in the F2 generation, a light green leaf individual plant with excellent comprehensive agronomic traits was selected, and a genetically stable line was obtained by continuous selfing for 4 generations. It was test crossed with the Dian I type japonica CMS line Chunjiang 16A, and a light green leaf indica with strong recovery was selected. The japonica intermediate restorer line CH101.

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Abstract

The invention relates to the field of hybrid rice breeding, in particular to a breeding method for pale-green leaf three-line indica and japonica inter-subspecies hybrid rice and a breeding method fora sterile line and an indica and japonica intermediate-type restoring line. The first aim of the invention is to provide the breeding method for the pale-green leaf three-line indica and japonica inter-subspecies hybrid rice, and by means of the method, a novel variety of the three-line indica and japonica inter-subspecies hybrid rice with high yield, pale-green leaf colors and good color changing in the later period is obtained. The second aim of the invention is to provide the breeding method for the pale-green leaf three-line sterile line, and by means of the method, the pale-green leaf three-line sterile line with stable sterile characteristics, good blooming habits, high outcrossing rate and good plant leaf shapes is bred. The third aim of the invention is to provide the breeding method for the pale-green leaf indica and japonica intermediate-type restoring line, and by means of the method, the pale-green leaf indica and japonica intermediate-type restoring line with good plant leaf shapes, good agronomic characters and good restorative capacity is bred.

Description

technical field [0001] This application relates to the field of hybrid rice breeding, in particular to a method for breeding light green leaf three-line sterile lines, indica-japonica intermediary restorer lines and indica-japonica inter-subspecies hybrid rice. Background technique [0002] In recent years, hybrid rice has been widely planted in my country, and its large-scale promotion is mainly due to the use of heterosis. The yield of hybrid rice is significantly higher than that of conventional rice varieties. In terms of the utilization degree of heterosis, due to the large genetic distance between indica and japonica subspecies, the heterosis of indica-japonica inter-subspecies hybrid rice is obviously stronger than indica-indica or japonica-japonica cross, so its potential for increasing yield is huge. However, the growth period of most indica-japonica inter-subspecies hybrid rice is relatively long, and there are problems of late ripening and poor color change in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 华宇峰宋昕蔚林建荣吴明国
Owner CHINA NAT RICE RES INST