Molecular marker of gpr54 gene affecting reproductive performance of Jiaxing black pig sows and its application

Abstract

The invention discloses a GPR54 gene molecular marker affecting the reproductive performance of Jiaxing black pig sows and its application. The GPR54 gene was used as a candidate gene affecting pig reproductive traits, and Jiaxing black pigs were used as a test group. The polymorphism of GPR54 gene was detected by direct sequencing, and the correlation analysis was carried out on the reproductive performance indicators of sows. The results showed that T→C mutation and C→T mutation occurred at 3739bp and 3878bp of exon 2 of GPR54 gene, and T→C mutation occurred at 6789bp of exon 6, both of which had two genotypes of AB and BB. The allele B is the dominant allele, and the BB genotype is the dominant allele type. The association analysis showed that the B allele of the GPR54 gene had a tendency to increase the total litter size, live litter size, and newborn litter weight of primiparous and multiparous Jiaxing black pig populations, and the T3739C locus had a positive effect on the total population and primiparous population. The total litter size and live litter size had a very significant effect (P<0.01), and had a significant effect on the first-born litter weight of primiparous groups (P<0.05).

Description

technical field [0001] The invention belongs to the field of animal breeding, and in particular relates to a GPR54 gene molecular marker affecting the reproductive performance of Jiaxing black pig sows and an application thereof. Background technique [0002] Pig reproductive traits are important economic traits in the pig industry, but this trait is a low heritability trait, with a heritability of about 0.1. With conventional breeding methods, it is impossible to fundamentally improve pig reproductive traits. With the development of molecular biology, modern molecular breeding technology can effectively improve the reproductive traits of pigs by carrying out genetic marker-assisted selection on the main genes or related genes that affect the reproductive traits of sows. [0003] Wu Jingsheng et al detected 3 SNPs in the GPR54 gene in 3 herds of Xiaomeishan pigs, Fengjing pigs and Large White pigs, which are T245C in exon 1, T3295C in exon 5 and intron 2 A base C was inser...

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Problems solved by technology

However, there are few reports on the effect of GPR54 gene on the sexual development and reproductive performance of pigs

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