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Aflatoxin B1 content detection method based on a magnetic signal

A technology of aflatoxin and detection method, applied in the fields of hardware detection and data analysis, can solve the problems of large signal noise, long response time, low sensitivity, etc., and achieve the effects of improving measurement accuracy, short response time and easy operation.

Pending Publication Date: 2019-11-19
HENAN UNIVERSITY OF TECHNOLOGY
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  • Application Information

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Problems solved by technology

[0008] The purpose of the present invention is to provide a method for detecting the content of aflatoxin B1 based on magnetic signals, in order to solve the problems of the existing AFB1 content detection method such as complex operation, long reaction time, large signal noise and low sensitivity

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  • Aflatoxin B1 content detection method based on a magnetic signal
  • Aflatoxin B1 content detection method based on a magnetic signal
  • Aflatoxin B1 content detection method based on a magnetic signal

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Embodiment Construction

[0024] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0025] The invention provides a method for detecting the content of aflatoxin B1 based on magnetic signals. The superparamagnetic nanoparticles used in the invention are ferric iron tetroxide, such as figure 1 shown, including the following steps:

[0026] The first step: AFB1 antibody modified superparamagnetic nanoparticles.

[0027] 1) Cleaning: Take 2mg of carboxylated magnetic beads in a 5mL centrifuge tube, wash twice with 2mL PB (0.02mol / L, pH6.0), wash with 2mL MEST (0.05mol / L, pH6.0, 0.05% or 0.1% Tween-20) resuspended;

[0028] 2) Activation: ultrasonically disperse the magnetic beads, wash with MEST for 3 times, then add 1mg EDC and 1mg NHS respectively, activate at 25°C for 30min, and rotate at 15r / min to maintain the suspended state;

[0029] 3) Coupling: Magnetic separation, suction off the supernatant, wash once with 2mL PBS (0.02mol / L, pH7....

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Abstract

The invention relates to an aflatoxin B1 content detection method based on a magnetic signal. The aflatoxin B1 content detection method comprises hardware detection (an excitation magnetic field element and a detection element) and a data analysis method. An aflatoxin B1 antibody is modified on a marker (superparamagnetic nanoparticles) to prepare a magnetic immunochromatographic test strip for detecting aflatoxin B1; the test strip is placed in an excitation magnetic field, and a magnetic response signal is detected by a detection element to obtain response magnetic field data corresponding to the aflatoxin B1 sample content; a relation model of the aflatoxin B1 sample content and the response magnetic field is established by taking the response magnetic field data and the aflatoxin B1 sample content as the input and output of a neural network model respectively; and the content of a to-be-detected aflatoxin B1 sample is obtained according to the relation model. The content of the aflatoxin B1 sample can be quantitatively detected only by acquiring the response magnetic field. The method has the advantages of rapid detection, high sensitivity and accuracy, easy and convenient operation, and the like.

Description

technical field [0001] The invention belongs to the field of food and feed detection. Specifically, the present invention relates to a method for detecting the content of aflatoxin B1 based on magnetic signals, including hardware detection (exciting magnetic field elements, detection elements), and data analysis methods. Background technique [0002] Aflatoxins are mainly a group of highly toxic and carcinogenic secondary metabolites produced by Aspergillus flavs, Aspergillus parasiticus and Aspergillus nonius. Aflatoxins are harmful to humans and many This kind of animal shows strong toxicity and has obvious carcinogenic ability. Among all mycotoxins known at present, its toxicity, carcinogenicity, mutagenicity and teratogenicity all occupy the first place. Aflatoxin BI (AflatoxinB1, AFB1) widely exists in various agricultural products, food and feed, and seriously pollutes peanuts, rice, corn, wheat and other grain and oil products, which brings great harm to people's hea...

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Application Information

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IPC IPC(8): G01N33/558G01N33/569G06N3/04G06N3/08
CPCG01N33/558G01N33/56961G06N3/082G06N3/048
Inventor 牛群峰赵旭燕王莉毛志鑫周潼刘任波
Owner HENAN UNIVERSITY OF TECHNOLOGY