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Method of preparing active polypeptides from semen euryales protein powder, active polypeptides and application

A technology of active polypeptide and protein powder, which is applied in the field of food processing, can solve the problems of small protein configuration space and unfavorable protease hydrolysis, etc., and achieve high yield of antioxidant active polypeptide, controllable degree of enzymatic hydrolysis, and good hydrolysis Effect

Inactive Publication Date: 2019-11-22
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on Gorgon fruit peptides still has the following problems: (1) Most of the studies focus on the screening of proteases used for high yield Gorgon fruit peptides, and generally use a single enzyme to hydrolyze Gorgon fruit protein powder; (2) Gorgon fruit protein powder The protein configuration space is small, the structure is compact, and it has strong hydrophobicity, which is not conducive to the hydrolysis of protease

Method used

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  • Method of preparing active polypeptides from semen euryales protein powder, active polypeptides and application
  • Method of preparing active polypeptides from semen euryales protein powder, active polypeptides and application

Examples

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Embodiment 1

[0028] This embodiment provides a method for preparing active polypeptide from Gorgon seed protein powder, comprising the following steps:

[0029] (1) Deionized water extraction: Weigh Gorgon protein, add water to extract at a ratio of 1:10, extract twice at 4°C for 3 hours each time, centrifuge the extract for 10min (4000r / min), and the supernatant is Gorgon Peptide crude extract A;

[0030] (2) Alkali extraction: Weigh the crude extract A of step (1), add water to extract at a ratio of 1:10, adjust the pH to 9.0 with 1mol / L NaOH, extract twice at 4°C, 3h / time, extract Centrifuge for 10min (4000r / min), the supernatant is the Gorgon fruit polypeptide crude extract B;

[0031] (3) Acid extraction: Weigh the crude extract B described in step (2), add 300mL distilled water, adjust the pH to 3.0 with 1mol / L acetic acid, extract twice at 4°C, 3h / time, and centrifuge the extract 10min (4000r / min), the supernatant is the Gorgon fruit polypeptide crude extract C;

[0032] (4) Tris...

Embodiment 2

[0036] This embodiment provides a method for preparing active polypeptide from Gorgon seed protein powder, comprising the following steps:

[0037] (1) Deionized water extraction: Weigh Gorgon protein, add water to extract at a ratio of 1:20, extract at 5°C for 3 times, 4h each time, centrifuge the extract for 12min (5000r / min), and the supernatant is Gorgon Peptide crude extract A.

[0038] (2) Alkaline extraction: Weigh the crude extract A of step (1), add water to extract at a ratio of 1:15, adjust the pH to 9.0 with 3mol / L NaOH, and extract at 5°C for 3 times, 4h / time, extract Centrifuge for 12 minutes (5000r / min), and the supernatant is the Gorgon fruit polypeptide crude extract B.

[0039] (3) Acid extraction: Weigh the crude extract B described in step (2), add 400mL distilled water, adjust the pH to 4.0 with 3mol / L acetic acid, extract 3 times at 5°C, 4h / time, and centrifuge the extract 12min (5000r / min), the supernatant is the Gorgon fruit polypeptide crude extract ...

Embodiment 3

[0044] This embodiment provides a method for preparing active polypeptide from Gorgon seed protein powder, comprising the following steps:

[0045] (1) Deionized water extraction: Weigh Gorgon protein, add water to extract at a ratio of 1:30, extract 3 times at 8°C, 5h each time, centrifuge the extract for 15min (6000r / min), and the supernatant is Gorgon Peptide crude extract A.

[0046] (2) Alkali extraction: Weigh the crude extract A of step (1), add water to extract at a ratio of 1:20, adjust the pH to 9.0 with 5mol / L NaOH, extract 4 times at -8°C for 5h each time, and extract Centrifuge for 15 minutes (6000r / min), and the supernatant is the Gorgon fruit polypeptide crude extract B.

[0047] (3) Acid extraction: Weigh the crude extract B described in step (2), add 500mL distilled water, adjust the pH to 5.0 with 5mol / L acetic acid, extract 4 times at 8°C, 5h / time, and centrifuge the extract 15min (6000r / min), the supernatant is the Gorgon fruit polypeptide crude extract C...

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Abstract

The invention relates to a method of preparing active polypeptides from semen euryales protein powder, the active polypeptides and application. The method includes the following steps of 1, extractionwith deionized water, 2, alkali extraction, 3, acid extraction, 4, Tris-HCl extraction, 5, alcohol extraction, and 6, enzymolysis. The prepared semen euryales active polypeptides are used for preparing food, healthcare food and specific food with medical-use formulas. Under technological conditions of the method, the semen euryales protein hydrolysis degree reaches 45.7%, the yield of the polypeptides is 37.9%, the hydroxyl radical removing rate of hydrolysis products is 65.1%, and the superoxide anion radical removing rate is 45%. A production technology is easy to operate and mild in condition, semen euryales hydrolysis conditions are good, the yield of the antioxidant active polypeptides is high, the enzymolysis degree is controllable, and an effective reference is provided for deep processing and comprehensive utilization of semen euryales protein.

Description

technical field [0001] The invention belongs to the technical field of food processing, and relates to a method for preparing active polypeptides from Gorgon seed protein powder, the active polypeptides and applications. Background technique [0002] Biologically active peptides refer to biologically active peptide substances obtained through protein degradation. Their molecular structures vary in complexity. They can be as simple as dipeptides consisting of only two amino acids, or complex ring-shaped macromolecular polypeptides. , Polypeptides have special physiological functions that proteins and amino acids that make up polypeptides do not necessarily have. Moreover, many polypeptides with high biological activity are not necessarily all composed of essential amino acids, making the amino acid composition like Gorgon protein powder not very ideal, the composition of essential amino acids is not very comprehensive, and is usually considered as a non-high-quality protein ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14
CPCC12P21/06C07K1/145
Inventor 刘志伟李明雷媛媛张挽挽王晓梦
Owner WUHAN POLYTECHNIC UNIVERSITY
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