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Method of determining acid hydrolysis rate of chitosan by ultraviolet spectrophotometry

A spectrophotometry, chitosan technology, applied in the direction of color/spectral property measurement, etc., can solve the problems of low yield of hydrolyzate, low hydrolysis efficiency, poor quality, etc.

Inactive Publication Date: 2018-07-06
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of acid-hydrolyzed chitosan under the protection of nitrogen with high efficiency, environmental protection and high yield aiming at the disadvantages of low yield, low hydrolysis efficiency and poor quality of the existing chitosan hydrolyzate Methods

Method used

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  • Method of determining acid hydrolysis rate of chitosan by ultraviolet spectrophotometry
  • Method of determining acid hydrolysis rate of chitosan by ultraviolet spectrophotometry
  • Method of determining acid hydrolysis rate of chitosan by ultraviolet spectrophotometry

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Embodiment 1

[0060] A method utilizing ultraviolet spectrophotometry to measure chitosan acid hydrolysis rate, it may further comprise the steps:

[0061] 1) Weigh 300mg of chitosan and add to the flask, measure 40ml of 6mol / L hydrochloric acid solution into the flask, seal the device, exhaust the air with a vacuum pump, and then feed nitrogen, repeat 3 times. Start the constant temperature magnetic stirrer, control the reaction temperature to 100°C, the rotation speed to 200r / min, and the reaction time to 48h. Use a sampling needle to take out an appropriate amount of hydrolyzate and seal it for storage as a sample, and place it in the refrigerator for later use;

[0062] 2) Determination of the content of glucosamine by ultraviolet spectrophotometry:

[0063] a) Draw the standard curve of A and different concentrations of glucosamine standard solution: draw 1.5mL of glucosamine standard solution with a certain concentration gradient into a 10mL colorimetric tube and dilute to 2mL with wa...

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Abstract

The invention relates to a method of determining an acid hydrolysis rate of chitosan by ultraviolet spectrophotometry and belongs to the technical field of macromolecular hydrolysis. The method takesa yield of GAH (D-glucosamine hydrochloride) in a chitosan hydrolysate as an index; a chitosan hydrolysis condition is optimized in a nitrogen protection environment; the hydrolysis condition is screened by an acetylacetone method; the best hydrolysis condition is preferred; a hydrolysis generation product, glucosamine, of the chitosan is verified; and a content of glucosamine is determined by theultraviolet spectrophotometry. The method has the characteristics of low cost, simple technology and high yield, and industrialization prospects.

Description

technical field [0001] The invention relates to a method for measuring the acid hydrolysis rate of chitosan by ultraviolet spectrophotometry, and belongs to the technical field of macromolecular hydrolysis. Background technique [0002] Chitin is a component of crustaceans such as shrimps and crabs, the exoskeleton of beetles, and the cell walls of fungi such as mushrooms, and is widely found in nature. Chitosan (chitosan) is the product of deacetylation of chitin. It is one of the few, natural and commercial alkaline polysaccharides, and its resource amount is second only to cellulose. Because chitosan has good film-forming properties, biodegradability, and certain antibacterial, anti-inflammatory and wound healing effects, it is widely used in many fields such as textiles, printing and dyeing, papermaking, food, medicine, and environmental protection. In terms of medical treatment, it can be used to make surgical lines, artificial blood vessels and artificial skin, hemost...

Claims

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Application Information

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IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 白研苏政权毋福海
Owner GUANGDONG PHARMA UNIV
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