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Correction device and correction method of temperature calibration device of PCR instrument

A calibration device and calibration device technology, which is applied in thermometer testing/calibration, thermometers, measuring devices, etc., can solve the problems that the calibration device cannot respond to the temperature, the temperature calibration of the PCR instrument is impossible, and the temperature sensor probe distortion is easy to promote. The effect of using, simple structure, and reducing the use of materials

Pending Publication Date: 2019-11-22
NAT INST OF METROLOGY CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether it is a thermocouple probe, a thermal resistance probe, or a composite probe, the temperature-potential relationship is not linear, but a fitting curve, and with the long-term use of the probe and the resulting inevitable Minor changes in the hardware, resulting in a deviation between the actual temperature-potential relationship and the preset fitting curve, causing the distortion of these temperature sensor probes, and finally causing the entire set of calibration devices to fail to reflect the real temperature situation, and it is even more impossible to test the PCR instrument. temperature calibration

Method used

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  • Correction device and correction method of temperature calibration device of PCR instrument
  • Correction device and correction method of temperature calibration device of PCR instrument
  • Correction device and correction method of temperature calibration device of PCR instrument

Examples

Experimental program
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Embodiment 1

[0040] Such as figure 1 As shown, the calibration device of the present invention includes a base 1 and a top cover 2 matched with the base 1 . Preferably, the opposite surfaces of the top cover and the base are rectangular, and the two are detachably connected by screws.

[0041] see further figure 2 A fixing device 3 for the temperature sensor probe is provided on the side of the base 1 pointing to the top cover 2, so as to fix the temperature sensor probe of the temperature calibration device for the PCR instrument. As a preferred solution, in order to ensure the best temperature conduction effect, the fixing device is blind holes 4 arranged in a matrix, and the spacing of blind holes 4 can be determined according to actual needs, for example, for a specific type of PCR instrument temperature calibration device , the spacing of the blind holes 4 is set to be the same as that of the temperature sensor probes of the calibration device, so as to achieve optimal matching. T...

Embodiment 2

[0045] Such as Figure 4 As shown, in order to facilitate the fixing of the data line of the PCR instrument temperature calibration device, on the basis of the calibration device shown in Embodiment 1, a hollow line-passing tube 7 matching the line-passing hole is further provided, and the data line passes from the accommodation space 5 Pass through the wire through-hole 6 and then wear the hollow wire-passing pipe 7. In order to further prevent the dissipation of heat, the correction device also includes a wire tube cover 8, which matches the opening of the hollow wire tube 7, for example, the outer diameter of the lower part of the wire tube cover 8 shrinks so that it can be inserted into the hollow in the wire-passing pipe 7. The wire-passing tube cover 8 is provided with a wire-passing slit 9 for the data line (usually a cable) of the PCR instrument temperature calibration device to pass through. As a preferred option, such as Figure 5 As shown, the wire passage slit 9...

Embodiment 3

[0047] On the basis of embodiment 1 or 2, this embodiment 3 demonstrates a preferred calibration method using the calibration device of the present invention.

[0048] S01. Put the PCR instrument temperature calibration device into the base 1 of the calibration device of the present invention, so that the temperature sensor probe of the PCR instrument temperature calibration device falls into the corresponding blind hole 4 .

[0049] S02. Pass the data line of the PCR instrument temperature calibration device through the line passing hole 6 of the top cover 2 of the calibration device of the present invention, in one embodiment through the line tube 7, and then buckle the top cover 2 Put it on the base 1 and close the cover.

[0050] S03. Put the calibration device into the oil tank of the precision temperature measuring device, put the standard platinum thermometer into the oil tank, and connect the PCR instrument temperature calibration device and the standard platinum therm...

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Abstract

The invention relates to a correction device and a correction method of a temperature calibration device of a PCR instrument. The device comprises a base and a top cover, and a fixing device is arranged on the face, pointing to the top cover, of the base, so that a temperature sensor probe of a temperature calibration device of a PCR instrument is fixed; the top cover is arranged above the base, and an accommodating space for accommodating the temperature calibration device of the PCR instrument is formed between the opposite surfaces of the top cover and the base; and the top cover is provided with a wire passing through hole on the opposite surface of the base, so that a data wire of the temperature calibration device of the PCR instrument penetrates out of the accommodating space. According to the correction device of a temperature calibration device of a PCR instrument, the accuracy of the temperature calibration device of the PCR instrument is ensured, so that the temperature accuracy of the PCR instrument calibrated by using the temperature calibration device of the PCR instrument is ensured, and the device is simple in structure, convenient to use, high in universality and convenient to popularize and use.

Description

technical field [0001] The invention belongs to the field of temperature detection and control, and in particular relates to a calibration device for a temperature calibration device (equipment) used in a PCR instrument, and further relates to a calibration method using the calibration device. Background technique [0002] Polymerase Chain Reaction (Polymerase Chain Reaction, PCR) is a molecular biology technique used to amplify specific DNA fragments in vitro in a short time, and is widely used in life science research, food hygiene, medical treatment, forensic medicine and environmental monitoring And many other aspects. The working principle of PCR instrument (gene amplification instrument) is to create different temperature environments for DNA amplification, denature DNA, anneal, and primer extension. Its essence is a precise temperature controller, and its temperature accuracy and consistency And fluctuation directly affect the amplification quality of gene fragments ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01K15/00
CPCG01K15/005
Inventor 高运华李春武利庆牛春艳王晶高颖
Owner NAT INST OF METROLOGY CHINA