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Analysis method for relieving nicotine-induced mouse sperm quantity and motility decline

A technology for sperm count and analysis method, applied in the field of medical experiments, can solve the problem of difficulty in finding the effect of Lycium barbarum polysaccharide on the decrease of sperm count and motility, and achieve the effects of alleviating the decrease in sperm count and motility, accurate data and uniform sperm distribution.

Pending Publication Date: 2019-11-26
CHANGZHI MEDICAL COLLEGE
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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem in the prior art that it is difficult to find the relationship between Lycium barbarum polysaccharides and the effect of nicotine-induced decline in sperm count and vitality in mice, and to provide an analysis method for alleviating the decline in sperm count and vitality in mice caused by nicotine. The mice were grouped into experiments, and the control variables were nicotine and Lycium barbarum polysaccharides. After the experiment was cultivated, the sperm activity of different groups of Kunming mice was analyzed and calculated, which facilitated the discovery of the relationship between Lycium barbarum polysaccharides and the alleviation of nicotine-induced decline in sperm motility, as well as the catsper gene, SOD1 and SOD2 gene play an important role in this process

Method used

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  • Analysis method for relieving nicotine-induced mouse sperm quantity and motility decline
  • Analysis method for relieving nicotine-induced mouse sperm quantity and motility decline
  • Analysis method for relieving nicotine-induced mouse sperm quantity and motility decline

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Embodiment 1

[0035] Refer to attached figure 1 As shown, a kind of analytical method of alleviating nicotine-induced decline in mouse sperm count and motility in the present embodiment, through grouping experiments with Kunming mice, controlling the difference variables to be nicotine and Lycium barbarum polysaccharides, after the experiment was cultivated, different groups of Kunming Mouse sperm motility was analyzed and calculated.

[0036] The concrete steps of the method of the present embodiment are:

[0037] S100, experiment grouping, adult male Kunming mice are randomly divided into four groups, each group has ten Kunming mice, and the four groups are A, B, C, and D respectively;

[0038] S200, experimental culture, four groups of experimental Kunming mice were fed with normal saline, nicotine, Lycium barbarum polysaccharide, and nicotine+Lycium barbarum polysaccharide respectively;

[0039] S300, preparation of experimental samples, each group of Kunming mice were dissected, and ...

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Abstract

The invention relates to an analysis method for relieving nicotine-induced mouse sperm quantity and motility decline, which belongs to the technical field of pharmaceutical experiments. According to the analysis method, Kunming mice are subjected to grouping experiments; distinguishing variables are controlled to be nicotine and lycium barbarum polysaccharide, and the sperm motility of the Kunmingmice of different groups is analyzed and calculated after experimental cultivation, so that a relationship between lycium barbarum polysaccharide and alleviation of nicotine-induced sperm motility decline and the important effects of a catsper gene, a SOD1 gene and a SOD2 gene in the process can be found conveniently.

Description

technical field [0001] The invention belongs to the technical field of medical experiments, and specifically relates to an analysis method for alleviating the decrease in sperm count and vitality in mice caused by nicotine. Background technique [0002] It is a well-known fact that smoking is harmful to health. Around the world, 2.5 million people die from smoking every year. Smoking is the number one killer of human beings. Consciously developing the personal hygiene habit of not smoking is not only beneficial to health, but also a manifestation of noble public health ethics. In a smoking room, especially in an environment with closed doors and windows in winter, the room is not only full of carbon dioxide exhaled by the human body, but also carbon monoxide exhaled by the smoker, which can cause headaches, fatigue, and decreased work efficiency. In the cold smoke spit out by the smoker, the content of tar and nicotine is 1 times more than that of the hot smoke inhaled by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50A61K31/715A61P15/00A61K49/00C12Q1/6888C12Q1/02
CPCA61K31/715A61K36/815A61K49/0008A61P15/00C12Q1/6888C12Q2600/158G01N33/5008
Inventor 宋少娟彭超张海娇田晓旭林恩琴谢翼马红莲刘明社
Owner CHANGZHI MEDICAL COLLEGE
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